A Study of Wnt/Wingless Signalling

9418793齐格弗里德这项建议的目的是了解细胞间通讯的机制，该机制调节果蝇黑腹果蝇胚胎的发育。Wingless是一种在胚胎中的一部分细胞中表达的蛋白质，它可以向不表达Wingless蛋白质的邻近细胞发出信号，并调节它们的命运。这种细胞信号传递的机制尚不清楚，但我们已经确定了一些其他参与信号传递途径的分子。泽斯特白3激酶是这一途径的一个组成部分，它能够通过向其他蛋白质中添加磷酸盐来修饰它们，并可能改变它们的活性。我们建议鉴定Zust White 3激酶确实修饰的蛋白质，并确定Zust White 3激酶活性本身是如何被调节的。对泽斯特·怀特3号的调查将使人们对无翼信号有更深入的了解。这与理解脊椎动物的生长和发育有关，因为Wingless与在老鼠身上发现的正常发育所需的蛋白质相似。此外，无翼信号通路在脊椎动物中可能是保守的，因为像Zust White 3这样的成分也有脊椎动物的对应成分。***

9418793 Siegfried The goal of this proposal is to understand the mechanism of cell- cell communication that regulates the development of the fruit fly Drosophila melanogaster embryo. Wingless is a protein that is expressed in a subset of cells in the embryo that can signal to neighboring cells that don't express the Wingless protein and regulate their fate. The mechanism of this cell signalling is unknown but we have identified a number of other molecules that are involved in the signalling pathway. Zeste white 3 kinase, which has the ability to modify other proteins by adding phosphate to them, and potentially alter their activity, is one component of this pathway. We propose to identify the proteins which Zeste white 3 kinase does modify and to determine how Zeste white 3 kinase activity is itself modulated. The investigation of Zeste white 3 will lead to a greater understanding of the Wingless signalling. This is relevant to the understanding of vertebrate growth and development since Wingless is similar to proteins found in mice that are required for normal development. In addition, the Wingless signalling pathway is likely to be conserved in vertebrates since components such as Zeste white 3 also have vertebrate counterparts. ***