The Kallikrein-kinin System in Non-tetrapod Vertebrates

非四足脊椎动物中的激肽释放酶-激肽系统

• 批准号: 9418819
• 负责人: J. Michael Conlon
• 金额: $ 11.24万
• 依托单位: Creighton University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-04-01 至 1999-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9418819&HistoricalAwards=false
• 关键词: Kallikrein; kinin; System; Non; tetrapod

9418819 Conlon In mammals, including the human, specific enzymes (kallikreins) cleave a circulating protein (kininogen) to generate a small peptide (bradykinin) that is involved in regulating blood flow and both neuronal and smooth muscle activity. This process is referred to as the kallikrein-kinin system but it is not known when the system arose in evolution or whether it plays an important functional role in non-mammalian vertebrates. Dr. Conlon proposes to examine plasma samples from a wide range of aquatic vertebrates at different levels of evolution for the presence of the components of the system. He has already purified novel bradykinin-related peptides from the trout and from the phylogentically ancient fish, the bowfin, and he proposes to systematically investigate the biological activities of the peptides in their species of origin. Dr. Conlon will study effects on cardiovascular parameters using chronically cannulated animals and identify target sites for the peptides using isolated tissue preparations. The data will permit an assessment of the physiological importance of the kallikrein-kinin system in fish. ***

在包括人类在内的哺乳动物中，特定的酶（激肽酶）会裂解循环蛋白（激肽原），产生一种小肽（缓激肽），这种肽参与调节血液流动以及神经元和平滑肌的活动。这一过程被称为激肽-激肽系统，但目前尚不清楚该系统是何时在进化中出现的，也不知道它是否在非哺乳动物脊椎动物中起着重要的功能作用。Conlon博士建议从各种不同进化水平的水生脊椎动物的血浆样本中检测该系统成分的存在。他已经从鳟鱼和从系统发育上古老的鱼中纯化了新的缓激肽相关肽，他建议系统地研究这些肽在其起源物种中的生物活性。Conlon博士将使用长期插管动物研究对心血管参数的影响，并使用分离组织制剂确定肽的靶点。这些数据将允许对鱼体内钾激肽-激肽系统的生理重要性进行评估。＊＊＊