Instrument Development for Three-Dimensional Fluorescence Microscopy

三维荧光显微镜仪器的开发

• 批准号: 9419342
• 负责人: Ting-Chung Poon
• 金额: $ 3.79万
• 依托单位: Virginia Polytechnic Institute and State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-04-15 至 1996-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9419342&HistoricalAwards=false
• 关键词: Instrument; Development; Three; Dimensional; Fluorescence

This proposal requests support for the purchase and development of several pieces of instrument which will be used to significantly advance a current research effort supported by the National Institutes of Health in the area of novel three-dimensional (3-D) microscopy. Specifically, the 3-D microscopy system currently being investigated will be developed into a high-resolution fluorescence microscopy system with real-time reconstruction capability by acquiring an Argon laser, an x-y computer-controlled scanning system, and a workstation. The Argon laser at 470 nm extends the microscopy system to be able to operate in a fluorescent mode, thereby potentially making the system a very useful and powerful tool in many branches of biology and the life sciences. The x-y scanning system allows the microscopy system to perform specimen-scanning instead of optical beam-scanning, thereby ensuring that the resolution and contrast are identical across the entire field of view. The workstation will improve the speed of the analysis of the 3-D data obtained from the microscopy system by an order of magnitude, thereby making it possible for real-time 3-D data manipulation and image presentation. The results of the proposed instrumentation development activity could lead to better 3-D information processing in areas such as real-time image processing, microscopy, and data storage and acquisition in the life sciences.

该提案要求支持购买和开发几件仪器，这些仪器将用于显著推进美国国立卫生研究院在新型三维（3-D）显微镜领域支持的当前研究工作。 具体而言，目前正在研究的3-D显微镜系统将发展成为一个高分辨率的荧光显微镜系统，通过获取氩激光，x-y计算机控制的扫描系统，和一个工作站的实时重建能力。470 nm的氩激光器扩展了显微镜系统，使其能够在荧光模式下操作，从而有可能使该系统成为生物学和生命科学许多分支中非常有用且强大的工具。x-y扫描系统允许显微镜系统执行双光束扫描而不是光束扫描，从而确保分辨率和对比度在整个视场中相同。该工作站将提高从显微镜系统获得的3-D数据的分析速度一个数量级，从而使实时3-D数据处理和图像呈现成为可能。拟议的仪器开发活动的结果可能会导致更好的三维信息处理领域，如实时图像处理，显微镜和数据存储和获取在生命科学。