Regulation and Assembly of Plant Pyruvate Dehydrogenase Complex

植物丙酮酸脱氢酶复合物的调控和组装

• 批准号: 9419489
• 负责人: Douglas Randall
• 金额: $ 27.75万
• 依托单位: University of Missouri-Columbia
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-02-01 至 1999-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9419489&HistoricalAwards=false
• 关键词: Regulation; Assembly; Plant; Pyruvate; Dehydrogenase

9419489 Randall A major point of interaction between photosynthesis, photorespiration and mitochondrial respiration is at the pyruvate dehydrogenase complex (PDC). This multi-enzyme complex is the primary entry point for carbon into the Krebs cycle for energy production and the generation of biosynthetic intermediates. PDC also provides the precursor for fatty acid biosynthesis, branch chain amino acids, isoprenoid compounds and some growth regulators. Objectives of this research are: to characterize the expression of PDC component enzymes, their mitochondrial import, processing and assembly in the complex; to characterize events, mechanisms and modulators of PDC activity during development and photosynthesis in C3, C4, and CAM plants; to extend biochemical characterization of PDC and establish in vitro and in organello regulatory mechanisms; and, to examine the long-term regulation/expression of PDC. Pea and Arabidopsis are the primary experimental systems used. This project will facilitate evaluation of the following concepts: that import and assembly of the mitochondrial matrix multi-enzyme cmplex, PDC, requires chaperones; that reversible phosphorylation of PDC is a primary control mechanism for carbon entry into the Krebs cycle; that PDC regulatory mechanisms differ during development and between C3, C4 and CAM plants.

9419489 Randall光合作用、光呼吸和线粒体呼吸之间的主要相互作用点在丙酮酸脱氢酶复合物（PDC）处。 这种多酶复合物是碳进入克雷布斯循环的主要入口点，用于能源生产和生物合成中间体的产生。 PDC还提供脂肪酸生物合成的前体、分支链氨基酸、类异戊二烯化合物和一些生长调节剂。 本研究的主要目的是：研究PDC组分酶的表达、线粒体的输入、加工和组装，研究C3、C4和CAM植物发育和光合作用过程中PDC活性的事件、机制和调控因子，扩展PDC的生化特性，建立PDC的体外和细胞器内调控机制，研究PDC在植物体内的生物学行为，为PDC的研究提供理论基础。以及检测PDC的长期调节/表达。 豌豆和拟南芥是使用的主要实验系统。 该项目将促进以下概念的评估：进口和组装的线粒体基质多酶复合物，PDC，需要分子伴侣;可逆磷酸化的PDC是一个主要的控制机制，碳进入克雷布斯循环; PDC的监管机制不同，在发展过程中和C3，C4和CAM植物之间。