Regulation and Assembly of Pyruvate Dehydrogenase Complexes

丙酮酸脱氢酶复合物的调控和组装

基本信息

  • 批准号:
    0325656
  • 负责人:
  • 金额:
    $ 45万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Continuing Grant
  • 财政年份:
    2003
  • 资助国家:
    美国
  • 起止时间:
    2003-09-01 至 2007-08-31
  • 项目状态:
    已结题

项目摘要

Respiration is the use of energy by living cells to do work. Both growth and reproduction are affected by respiration and it must be carefully controlled to avoid decreased growth and, in the case of plants, reduced agricultural productivity. Despite considerable research, details of how respiration is controlled in plant cells remain a puzzle. Pyruvate dehydrogenase is a multi-component enzyme complex located within a specific sub-cellular compartment of plant cells. It occupies a cross-roads position where there is interaction among multiple components of respiration. This complex is ideally situated to play a major role in the overall control of respiration. Furthermore, the multi-component architecture of the complex allows input from several different mechanisms. One element of the regulatory scheme may be an intrinsic component of the pyruvate dehydrogenase complex. Detailed biochemical and molecular analyses of this component will provide critical insight into the putative mechanism of control. In addition, a genetic strategy that uses whole plants will be employed. A small model plant, mouse-eared cress, will be used. Molecular genetic experiments will allow the complete elimination of the proposed control component of the pyruvate dehydrogenase complex. If the control hypothesis is correct, uncontrolled respiration will result in small, less-productive plants. These plants will be rescued by replacing the control component. Both the native control component and versions that have been modified in the laboratory will be used. This will allow specific understanding of the mechanism of control. In contrast to microbes or animals, plant cells contain several different versions of another component of the pyruvate dehydrogenase complex. Preliminary studies have shown that the situation is not so simple as having different versions functioning at different times or in different places. Again, the mouse-eared cress plant will be manipulated so as to eliminate two of the three versions of this component. These experiments will be iterative and combinatorial. This means that plants will be generated that contain only component 1, only 2, only 3, 1 and 2, 1 and 3, and 2 and 3. These manipulations will allow a better understanding of the contribution that each component makes to the whole complex. A third component of the pyruvate dehydrogenase complex has been an enigma. There is considerable evidence for its existence, but it has thus far not been isolated. Attempts to isolate this component by molecular genetics have not been successful. The return to a more classical biochemical isolation strategy will be undertaken. However it will be a more specific strategy based upon results from other plant and animal experimental systems. Isolation of this third component will allow subsequent isolation of the gene. Once this is accomplished, then the biochemical and molecular strategies described above will be applied. The information gained from these experiments will improve basic understanding of plant growth and development. Furthermore, there is the potential that the results will allow researchers to increase agricultural productivity by altering the control of plant cell respiration. Finally, the results will inform the design of more efficient crop plants through classical breeding or biotechnology.
呼吸是活细胞利用能量做功。 生长和繁殖都受到呼吸作用的影响,必须仔细控制呼吸作用,以避免植物生长减缓和农业生产力下降。 尽管有大量的研究,植物细胞中呼吸是如何控制的细节仍然是一个谜。丙酮酸脱氢酶是一种多组分酶复合物,位于植物细胞的特定亚细胞区室中。 它占据了一个十字路口的位置,那里有呼吸的多个组成部分之间的相互作用。 这种复合物的理想位置是在呼吸的整体控制中发挥重要作用。 此外,综合体的多组件架构允许来自几个不同机制的输入。调节方案的一个要素可以是丙酮酸脱氢酶复合物的内在组分。 详细的生化和分子分析,这一组成部分将提供关键的洞察力的推定机制的控制。 此外,将采用使用整株植物的遗传策略。 一个小的模型植物,鼠耳水芹,将被使用。 分子遗传学实验将允许完全消除丙酮酸脱氢酶复合物的拟定控制组分。 如果控制假说是正确的,不受控制的呼吸将导致小型,生产力较低的植物。 这些植物将通过更换控制组件来拯救。 将使用本地控件组件和在实验室中修改的版本。 这将有助于对控制机制的具体理解。与微生物或动物相反,植物细胞含有丙酮酸脱氢酶复合物的另一种组分的几种不同形式。 初步研究表明,这种情况并不像在不同时间或不同地点有不同的版本那么简单。 同样,鼠耳水芹植物将被操纵,以消除三个版本的这一组成部分的两个。 这些实验将是迭代和组合的。 这意味着将生成仅包含组分1、仅包含组分2、仅包含组分3、仅包含组分1和组分2、仅包含组分1和组分3以及仅包含组分2和组分3的植物。 这些操作将允许更好地理解每个组件对整个复杂系统的贡献。丙酮酸脱氢酶复合物的第三种成分一直是个谜。 有相当多的证据表明它的存在,但迄今为止还没有被孤立。 通过分子遗传学分离该组分的尝试尚未成功。 将采用更经典的生化隔离策略。 然而,这将是一个更具体的策略,基于其他植物和动物实验系统的结果。 分离该第三组分将允许随后分离基因。 一旦完成,则将应用上述生物化学和分子策略。从这些实验中获得的信息将提高对植物生长和发育的基本认识。 此外,研究结果有可能使研究人员通过改变植物细胞呼吸的控制来提高农业生产力。 最后,研究结果将为通过传统育种或生物技术设计更高效的作物提供信息。

项目成果

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Douglas Randall其他文献

Douglas Randall的其他文献

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{{ truncateString('Douglas Randall', 18)}}的其他基金

Regulation and Assembly of Pyruvate Dehydrogenase Complexes
丙酮酸脱氢酶复合物的调控和组装
  • 批准号:
    9876680
  • 财政年份:
    1999
  • 资助金额:
    $ 45万
  • 项目类别:
    Continuing Grant
Regulation and Assembly of Plant Pyruvate Dehydrogenase Complex
植物丙酮酸脱氢酶复合物的调控和组装
  • 批准号:
    9419489
  • 财政年份:
    1995
  • 资助金额:
    $ 45万
  • 项目类别:
    Continuing Grant
Regulation of the Pyruvate Dehydrogenase Complex in Photosynthetic and Developing Plant Tissue
丙酮酸脱氢酶复合物在光合作用和发育中植物组织中的调节
  • 批准号:
    9201292
  • 财政年份:
    1992
  • 资助金额:
    $ 45万
  • 项目类别:
    Standard Grant
Current Topics Symposia in Plant Biochemistry and Physiology, Columbia, Missouri, 1990, 1991, 1992
植物生物化学和生理学当前主题研讨会,哥伦比亚,密苏里州,1990、1991、1992
  • 批准号:
    9008099
  • 财政年份:
    1990
  • 资助金额:
    $ 45万
  • 项目类别:
    Standard Grant
Symposium: Current Topics in Plant Biochemistry and Physiology
研讨会:植物生物化学和生理学的当前主题
  • 批准号:
    8417789
  • 财政年份:
    1985
  • 资助金额:
    $ 45万
  • 项目类别:
    Continuing Grant
Pyruvate Dehydrogenase Complex in Photosynthetic and Developing Tissues
光合和发育组织中的丙酮酸脱氢酶复合物
  • 批准号:
    8506473
  • 财政年份:
    1985
  • 资助金额:
    $ 45万
  • 项目类别:
    Standard Grant
Pyruvate Dehydrogenase Complex in Photosynthetic and Developing Tissue
光合和发育组织中的丙酮酸脱氢酶复合物
  • 批准号:
    8104659
  • 财政年份:
    1982
  • 资助金额:
    $ 45万
  • 项目类别:
    Standard Grant
Pyruvate Dehydrogenase Complex in Photosynthetic Tissue
光合组织中的丙酮酸脱氢酶复合物
  • 批准号:
    7711390
  • 财政年份:
    1977
  • 资助金额:
    $ 45万
  • 项目类别:
    Continuing Grant
Pyruvate Dehydrogenase Complex in Photosynthetic Tissue
光合组织中的丙酮酸脱氢酶复合物
  • 批准号:
    7508107
  • 财政年份:
    1975
  • 资助金额:
    $ 45万
  • 项目类别:
    Standard Grant

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晶态桥联聚倍半硅氧烷的自导向组装(self-directed assembly)及其发光性能
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