Analysis of Oxidative DNA Repair Enzymes in Damaged Rat Kidney (Biology)

受损大鼠肾脏氧化 DNA 修复酶的分析（生物学）

• 批准号: 9450473
• 负责人: Patricia Gallagher
• 金额: $ 12.7万
• 依托单位: Wake Forest University School of Medicine
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-09-01 至 1995-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9450473&HistoricalAwards=false
• 关键词: Analysis; Oxidative; DNA; Repair; Enzymes

This research includes a study of two enzymes, the redoxyendonuclease and formamidopyrimidine/8-oxyguanine-DNA glycosylase, which initiate the repair of oxidative base damages in the DNA. The levels of both enzymes will be quantified by biochemical assays in extracts of rat kidney tissue subjected to physiological oxidant stress as compared to the undamaged kidney tissue from the same animal as well as untreated controls. The results of these studies will lead to a better understanding of the induction of DNA repair activities in response to oxidative DNA damage and provide a basis for future studies on the fundamental mechanisms underlying free radical-mediated damage in animal tissues. Interactive activities include: teaching a revised graduate level pharmacology course with emphasis on molecular biology principles and techniques; organizing a panel discussion focusing on the differences in which men and women conduct science; and offering a recombinant DNA technology short course to undergraduate and graduate students as well as interested faculty.

本研究包括两种酶的研究，氧化还原酶核酸内切酶和甲酰氨基嘧啶/8-氧鸟嘌呤-DNA糖基化酶，启动DNA中的氧化碱基损伤的修复。 将通过生物化学测定法定量两种酶的水平，并与来自相同动物的未受损肾组织以及未处理对照相比，对经受生理氧化应激的大鼠肾组织提取物进行定量。 这些研究的结果将导致更好地理解DNA修复活动的诱导，以应对氧化DNA损伤，并为未来的研究基础上的基本机制，在动物组织中的自由基介导的损伤。 互动活动包括：教授修订后的研究生水平的药理学课程，重点是分子生物学原理和技术;组织一次小组讨论，重点是男子和妇女从事科学的差异;并为本科生和研究生以及感兴趣的教师提供重组DNA技术短期课程。