Instrumentation for an Advanced Undergraduate Laboratory in Biochemistry and Molecular Biology

9451005蔡生物化学与分子生物学高级技术本科生精修实验室课程--高级生物化学与分子生物学实验室(Chem 5362)--是新开设的生物化学与分子生物学理科学士学位课程所要求的高级课程。该实验室的一个主要特点是，学生可以自己完成所有的程序，而不需要进行任何“演示”练习。所使用的练习将包括学生在该领域的职业生涯中可能会遇到的最先进的技术。这些技术包括计算机辅助DNA序列分析、聚合酶链式反应(PCR)、DNA电穿孔、同源重组、体外诱变、报告基因融合、在大肠杆菌中生产真核蛋白、自动低压高效液相色谱(HPLC)生物分子。这些技术的“实践”经验使学生在未来的工作中具有竞争优势。

9451005 Tsai An intensive laboratory course for undergraduates in advanced techniques of biochemistry and molecular biology -Advanced Biochemistry and Molecular Biology Laboratory (Chem 5362)-is a senior level course, required by the new Bachelor of Science program in Biochemistry and Molecular Biology. A key feature of the lab is that the students perform all procedures themselves without involving any "demonstration" exercises. Exercises used will include state-of-the-art techniques that the students are likely to encounter later in their careers in this field. These include computer-assisted DNA sequence analysis, polymerase chain reaction (PCR), DNA electroporation, homologous recombination, in vitro mutagenesis, reporter gene fusions, production of eukaryotic proteins in E. coli, automated low pressure liquid chromatography, and high pressure liquid chromatography (HPLC) of biological molecules. A "hands-on" experience with techniques such as these gives students a competitive edge in their future endeavors.