Equipment to Implement a New Undergraduate Biochemistry Laboratory Curriculum

两门课程的本科生物化学实验室序列以以问题为导向的长期项目取代了传统的以技术为导向的短期练习。这些项目阐述了生物化学和分子生物学的主要主题，同时教授方法基础和当前技术。第一门课程的重点是酶的分离和表征。学生将学习大分子纯化的基本策略，并熟悉硫酸铵分离、亲和层析、聚丙烯酰胺凝胶电泳、免疫印迹、凝胶排除层析和几种蛋白质检测方法的技术。他们还从事酶动力学的深入研究。第二门课程让学生了解原创性研究的本质。学生将根据该领域当前的研究文献，研究有关膜功能和基因调控的项目。

9452118 Martinson A two-course undergraduate Biochemistry laboratory sequence features problem-oriented long term projects in place of the traditional technique-oriented short-term exercises. The projects illustrate major themes in Biochemistry and Molecular Biology, while teaching methodological fundamentals as well as current techniques. The first course focuses on the isolation and characterization of an enzyme. Students learn the fundamental strategies for macromolecular purification, and become acquainted with the techniques of ammonium sulfate fractionation, affinity chromatography, polyacrylamide gel electrophoresis, immunoblotting, gel exclusion chromatography, and several means of protein detection. They also engage in a thorough study of enzyme kinetics. The second course acquaints students with the nature of original research. Students investigate projects dealing with membrane function and gene regulation, based directly on current research literature in the field.