Equipment to Implement a New Undergraduate Biochemistry Laboratory Curriculum

9452118马丁森一个两门课程的本科生物化学实验室序列，以问题为导向的长期项目取代传统的以技术为导向的短期练习。这些项目说明了生物化学和分子生物学的主要主题，同时教授方法学基础和当前的技术。第一门课程的重点是酶的分离和特性。学生学习大分子纯化的基本策略，并熟悉硫酸铵分级、亲和层析、聚丙烯酰胺凝胶电泳、免疫印迹、凝胶排斥层析和几种蛋白质检测方法的技术。他们还对酶的动力学进行了深入研究。第二门课程让学生了解原创研究的本质。学生直接根据该领域当前的研究文献，研究与膜功能和基因调控有关的项目。

9452118 Martinson A two-course undergraduate Biochemistry laboratory sequence features problem-oriented long term projects in place of the traditional technique-oriented short-term exercises. The projects illustrate major themes in Biochemistry and Molecular Biology, while teaching methodological fundamentals as well as current techniques. The first course focuses on the isolation and characterization of an enzyme. Students learn the fundamental strategies for macromolecular purification, and become acquainted with the techniques of ammonium sulfate fractionation, affinity chromatography, polyacrylamide gel electrophoresis, immunoblotting, gel exclusion chromatography, and several means of protein detection. They also engage in a thorough study of enzyme kinetics. The second course acquaints students with the nature of original research. Students investigate projects dealing with membrane function and gene regulation, based directly on current research literature in the field.