Equipment to Implement a New Undergraduate Biochemistry Laboratory Curriculum

小行星9452118 两门课程的本科生生物化学实验室序列 以问题为导向的长期项目代替传统的以技术为导向的短期练习。这些项目说明了生物化学和分子生物学的主要主题，同时教授方法论基础知识和当前技术。第一门课程着重于酶的分离和表征。学生学习大分子纯化的基本策略，并熟悉硫酸铵分级分离，亲和色谱，聚丙烯酰胺凝胶电泳，免疫印迹，凝胶排阻色谱和蛋白质检测的几种方法。他们还从事酶动力学的深入研究。 第二门课程使学生具有原创性研究的性质。学生调查处理膜功能和基因调控的项目，直接基于该领域当前的研究文献。

9452118 Martinson A two-course undergraduate Biochemistry laboratory sequence features problem-oriented long term projects in place of the traditional technique-oriented short-term exercises. The projects illustrate major themes in Biochemistry and Molecular Biology, while teaching methodological fundamentals as well as current techniques. The first course focuses on the isolation and characterization of an enzyme. Students learn the fundamental strategies for macromolecular purification, and become acquainted with the techniques of ammonium sulfate fractionation, affinity chromatography, polyacrylamide gel electrophoresis, immunoblotting, gel exclusion chromatography, and several means of protein detection. They also engage in a thorough study of enzyme kinetics. The second course acquaints students with the nature of original research. Students investigate projects dealing with membrane function and gene regulation, based directly on current research literature in the field.