Soluble Methane Monooxygenase Expression and Co-oxidation Activity under Nitrogen-Limited Conditions (Collaborative Research)

限氮条件下可溶性甲烷单加氧酶的表达和共氧化活性（合作研究）

• 批准号: 9504383
• 负责人: Alan Taylor
• 金额: $ 9.64万
• 依托单位: Wichita State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-01-01 至 1996-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9504383&HistoricalAwards=false
• 关键词: Soluble; Methane; Monooxygenase; Expression; Co

9504383 Taylor This project is on research to improve the bioremediation of halogenated compounds by increasing the expression and activity of the soluble methane monooxygenase (sMMO) enzyme in methanotrophic bacteria. The strategy is to culture wild-type and mutant strains of bacteria in various copper, nitrogen, and oxygen environments, while monitoring sMMO and nitrogenase expression and activity. The co-oxidation rates of a variety of environmental pollutants will be quantified for selected strains, with attention to the influence of nitrogen limitation. These pollutants include halogenated ethanes, halomethanes and selected pesticides. Finally, the expression and activity patterns will be related to the environmental conditions by a model which will be used to develop reactor designs. ***

9504383 Taylor该项目是关于通过增加甲烷氧化细菌中可溶性甲烷单加氧酶（sMMO）的表达和活性来改善卤代化合物的生物修复的研究。 该策略是在各种铜、氮和氧环境中培养野生型和突变型细菌菌株，同时监测sMMO和固氮酶的表达和活性。 各种环境污染物的共氧化率将被量化为选定的菌株，注意氮限制的影响。 这些污染物包括卤代乙烷、卤代甲烷和选定的杀虫剂。 最后，表达和活动模式将与环境条件的模型，将用于开发反应堆设计。 ***