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Soluble Methane Monooxygenase Expression and Co-oxidation Activity under Nitrogen-Limited Conditions (Collaborative Research)

Soluble Methane Monooxygenase Expression and Co-oxidation Activity under Nitrogen-Limited Conditions (Collaborative Research)
限氮条件下可溶性甲烷单加氧酶的表达和共氧化活性（合作研究）
批准号：
9504383
负责人：
Alan Taylor
金额：
$9.64万
依托单位：
Wichita State University
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
1995
资助国家：
美国
项目状态：
已结题
起止时间：
1995-01-01 至 1996-12-31
来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=9504383&HistoricalAwards=false
关键词：
SolubleMethaneMonooxygenaseExpressionCo

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英文摘要
9504383 Taylor This project is on research to improve the bioremediation of halogenated compounds by increasing the expression and activity of the soluble methane monooxygenase (sMMO) enzyme in methanotrophic bacteria. The strategy is to culture wild-type and mutant strains of bacteria in various copper, nitrogen, and oxygen environments, while monitoring sMMO and nitrogenase expression and activity. The co-oxidation rates of a variety of environmental pollutants will be quantified for selected strains, with attention to the influence of nitrogen limitation. These pollutants include halogenated ethanes, halomethanes and selected pesticides. Finally, the expression and activity patterns will be related to the environmental conditions by a model which will be used to develop reactor designs. ***
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