Thermodynamic and Molecular Mechanisms of Protein Stabilization by Polymers During Freezing, Drying, and Rehydration: EPR and FTIR Studies

冷冻、干燥和再水合过程中聚合物稳定蛋白质的热力学和分子机制：EPR 和 FTIR 研究

• 批准号: 9505301
• 负责人: Theodore Randolph
• 金额: $ 39.87万
• 依托单位: University of Colorado at Boulder
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-09-01 至 1998-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9505301&HistoricalAwards=false
• 关键词: Thermodynamic; Molecular; Mechanisms; Protein; Stabilization

9505301 Randolph This project is on research to better understand the mechanisms for polymer-induced protein stabilization in solution during freezing and drying. The research will focus on the thermodynamics and molecular interactions of polymeric additives with proteins in aqueous solutions, frozen solutions, and dried solids, and how these interactions dictate protein stability. In particular this includes: (1) solution thermodynamics which involves phase behavior and protein/polymer preferential interactions; and (2) experimental measurements which include differential scanning calorimetry, electron paramagnetic resonance spectroscopy (EPR) and resolution-enhanced Fourier transform infrared spectroscopy (FTIR). This will be used to determine why polymers work and why they fail as protein stabilizers. ***

9505301伦道夫这个项目是为了更好地了解聚合物诱导蛋白质在冷冻和干燥过程中在溶液中稳定的机制而进行的研究。这项研究将集中在聚合物添加剂与蛋白质在水溶液、冷冻溶液和干燥固体中的热力学和分子相互作用，以及这些相互作用如何决定蛋白质的稳定性。具体包括：(1)溶液热力学，包括相行为和蛋白质/聚合物优先相互作用；(2)实验测量，包括差示扫描量热法、电子顺磁共振光谱(EPR)和分辨率增强型傅里叶变换红外光谱(FTIR)。这将被用来确定聚合物为什么起作用，以及为什么它们作为蛋白质稳定剂失败。***