Elucidating the Molecular Mechanism of a Ni Hydrogenase: An Infrared Spectroscopic Approach
Elucidating the Molecular Mechanism of a Ni Hydrogenase: An Infrared Spectroscopic Approach
批准号:
9509064
负责人:
Kimberly Bagley
金额:
$1.8万
依托单位国家:
美国
项目类别:
Standard Grant
财政年份:
1995
资助国家:
美国
项目状态:
已结题
起止时间:
1995-06-15 至 1997-11-30
中文摘要
点击翻译按钮获取中文摘要
英文摘要
9509064 Bagley This research details the utilization of static and time resolved Fourier transform infrared spectroscopy for delineating the molecular mechanism of hydrogen binding and activation in Ni hydrogenases. The research objectives are: (1) Determination of whether the anomalous infrared bands detected between 2100 and 1900 cm-1 are general features of Ni hydrogenases. To this end a series of infrared studies will be carried out on a well-characterized hydrogenase from Desulfovibrio gigas. (2) Identification of the structure responsible for these unusual infrared signatures using infrared spectroscopy coupled with chemical modification of the enzyme and the study of isotopically labeled enzyme. (3) Utilization of the three infrared signatures for further study of the various redox states of the enzyme. This approach provides a unique opportunity; Ni hydrogenases display at least two EPR-silent species which are distinguishable by their infrared signatures in the 2100 and 1900 cm-1 spectral region. (4) Utilization of mid- infrared spectroscopy to examine the role individual amino acids play in the activation of hydrogen. (5) Examination of the oxidation state of the Ni center in various redox states of the enzymes. This approach used the infrared detectable carbon monoxide stretching frequency, arising from Ni bound carbon monoxide, as a probe of the oxidation state of the Ni. %%% Ni Hydrogenases are enzymes that catalyze the simplest reaction in nature and the simplest conversion of electrical energy into chemical energy of universal utility: 2e- + 2H+ = H2. This research involves a spectroscopic study of the anomalous infrared absorption bands that are characteristic of these enzymes. Infrared spectroscopic studies will be made on the well-characterized Ni hydrogenases from Desulfovibrio gigas. It is a anticipated that this work will have important consequences for understanding the mechanism underlying Ni hydrogenase's ability to split molecular hydrogen into protons and electrons. The work also involves the development of methodologies for handling infrared samples under strictly anaerobic conditions, and should prove of general utility in infrared studies of other oxygen sensitive metallo-proteins. ***
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Acquisition of a Step-scan Time-resolved FTIR and pulsed Nd-YAG Pump Laser for Studies of Photo-induced Species in Chemical and Biomolecular Systems
-
批准号:0520826
-
项目类别:Standard Grant
-
资助金额:$26.57万
-
财政年份:2005
-
负责人:Kimberly Bagley
-
依托单位:
RUI: Infrared Spectroscopy as a Probe of Active Site Structure and Molecular Mechanism in the Hydrogenases
-
批准号:0130905
-
项目类别:Continuing Grant
-
资助金额:$21.0万
-
财政年份:2002
-
负责人:Kimberly Bagley
-
依托单位:
RUI: Infrared Spectroscopy as a Probe of Molecular Mechanismin the Hydrogenases
-
批准号:9723828
-
项目类别:Standard Grant
-
资助金额:$15.0万
-
财政年份:1997
-
负责人:Kimberly Bagley
-
依托单位:
PostDoctoral Research Fellowships in Plant Biology
-
批准号:8710654
-
项目类别:Fellowship Award
-
资助金额:$5.28万
-
财政年份:1987
-
负责人:Kimberly Bagley
-
依托单位:
国内基金
海外基金
登录
查看更多内容
Kidney injury molecular(KIM-1)介导肾小管上皮细胞自噬在糖尿病肾病肾间质纤维化中的作用
-
批准号:81300605
-
项目类别:青年科学基金项目
-
资助金额:23.0万元
-
批准年份:2013
-
负责人:唐琳
-
依托单位:
Molecular Plant
-
批准号:31224801
-
项目类别:专项基金项目
-
资助金额:20.0万元
-
批准年份:2012
-
负责人:黄健秋
-
依托单位:
Molecular Interaction Reconstruction of Rheumatoid Arthritis Therapies Using Clinical Data
-
批准号:31070748
-
项目类别:面上项目
-
资助金额:34.0万元
-
批准年份:2010
-
负责人:Christine Nardini
-
依托单位:
Molecular Plant
-
批准号:31024802
-
项目类别:专项基金项目
-
资助金额:20.0万元
-
批准年份:2010
-
负责人:陈晓亚
-
依托单位:
Cellular & Molecular Immunology
-
批准号:30824806
-
项目类别:专项基金项目
-
资助金额:20.0万元
-
批准年份:2008
-
负责人:魏海明
-
依托单位: