RUI: Infrared Spectroscopy as a Probe of Active Site Structure and Molecular Mechanism in the Hydrogenases

RUI:红外光谱作为氢化酶活性位点结构和分子机制的探针

基本信息

  • 批准号:
    0130905
  • 负责人:
  • 金额:
    $ 21万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Continuing Grant
  • 财政年份:
    2002
  • 资助国家:
    美国
  • 起止时间:
    2002-03-01 至 2006-08-31
  • 项目状态:
    已结题

项目摘要

In this project infrared difference spectroscopy will be used to study the active site structure and molecular mechanism of hydrogen binding and activation in the hydrogenases. Previous infrared spectroscopic studies have shown that both the [NiFe] hydrogenases and the Fe-only hydrogenases display intense infrared bands in the 2100-1800 cm-1 spectral region. The groups responsible for these infrared bands have been identified as arising from intrinsic carbon monoxide and cyanide molecules coordinated to Fe ions in the active site of these enzymes. The primary objectives of this research are: 1) Utilization of infrared spectroscopy for delineating the active site structure of the [NiFe] hydrogenase from Desulfovibrio vulgaris. The enzyme from Desulfovibrio vulgaris has been proposed to have a NiFe (CO)(CN)(SO) cluster at its active site rather than the NiFe (CO)(CN)2 cluster shown to exist in other [NiFe] Hydrogenases. 2) Utilization of infrared difference spectroscopy to explore the molecular changes accompanying reductive activation in the [NiFe] hydrogenases. 3) Utilization of the infrared spectral signatures, combined with isotopic labeling, for determining the active site structure of Fe-only Hydrogenases I and II from Clostridium pasteurianum. 4) Utilization of mid-infrared difference spectroscopy to examine the role amino acids play in the activation of hydrogen. The research will involve undergraduate students pursuing degrees in chemistry and chemistry-related fields.It is anticipated that this work will have important consequences for understanding the mechanism underlying the hydrogenases' ability to split molecular hydrogen into protons and electrons, or conversely, to produce hydrogen from protons and electrons. Such knowledge has consequences for the design of cheap catalysts for the production of hydrogen, a clean burning fuel source. The proposed research will involve the participation of undergraduate students, both during the academic year and during the summer. Student participation in this project will afford undergraduate students a unique opportunity to apply skills and knowledge they have learned in their coursework, while contributing to an exciting project in modern physical biochemistry.
本计画将利用红外差光谱技术来研究氢化酶的活性中心结构以及氢结合与活化的分子机制。 以前的红外光谱研究表明,无论是[NiFe]氢化酶和Fe-唯一的氢化酶显示在2100-1800 cm-1光谱区域的强红外波段。 负责这些红外波段的基团已被确定为来自内在的一氧化碳和氰化物分子在这些酶的活性位点中与Fe离子配位。 本研究的主要目的是:1)利用红外光谱技术研究脱硫弧菌[NiFe]氢化酶的活性中心结构。来自普通脱硫弧菌的酶已经被提出在其活性位点具有NiFe(CO)(CN)(SO)簇,而不是在其他[NiFe]氢化酶中显示存在的NiFe(CO)(CN)2簇。2)利用红外差光谱研究[NiFe]氢化酶中还原活化过程中的分子变化。 3)利用红外光谱特征,结合同位素标记,用于确定来自巴氏梭菌的仅含Fe的氢化酶I和II的活性位点结构。 4)利用中红外差光谱研究氨基酸在氢活化中的作用。研究对象为化学及化学相关专业的本科生,预计对于理解氢化酶将氢分子分解为质子和电子,或者反过来将质子和电子转化为氢的机理具有重要意义。这些知识对于设计廉价的催化剂来生产氢(一种清洁的燃烧燃料来源)具有重要意义。 拟议的研究将涉及本科生的参与,无论是在学年和夏季。 学生参与这个项目将为本科生提供一个独特的机会,运用他们在课程中学到的技能和知识,同时为现代物理生物化学中令人兴奋的项目做出贡献。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Kimberly Bagley其他文献

Kimberly Bagley的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Kimberly Bagley', 18)}}的其他基金

Acquisition of a Step-scan Time-resolved FTIR and pulsed Nd-YAG Pump Laser for Studies of Photo-induced Species in Chemical and Biomolecular Systems
获取步进扫描时间分辨 FTIR 和脉冲 Nd-YAG 泵浦激光器,用于研究化学和生物分子系统中的光诱导物质
  • 批准号:
    0520826
  • 财政年份:
    2005
  • 资助金额:
    $ 21万
  • 项目类别:
    Standard Grant
RUI: Infrared Spectroscopy as a Probe of Molecular Mechanismin the Hydrogenases
RUI:红外光谱作为氢化酶分子机制的探针
  • 批准号:
    9723828
  • 财政年份:
    1997
  • 资助金额:
    $ 21万
  • 项目类别:
    Standard Grant
Elucidating the Molecular Mechanism of a Ni Hydrogenase: An Infrared Spectroscopic Approach
阐明镍氢化酶的分子机制:红外光谱方法
  • 批准号:
    9509064
  • 财政年份:
    1995
  • 资助金额:
    $ 21万
  • 项目类别:
    Standard Grant
PostDoctoral Research Fellowships in Plant Biology
植物生物学博士后研究奖学金
  • 批准号:
    8710654
  • 财政年份:
    1987
  • 资助金额:
    $ 21万
  • 项目类别:
    Fellowship Award

相似国自然基金

基于局部视觉关联的RGB-Infrared物体检测
  • 批准号:
  • 批准年份:
    2022
  • 资助金额:
    30 万元
  • 项目类别:
    青年科学基金项目

相似海外基金

RUI: Probing the interplay between magnetism and relativistic fermions in the Weyl semimetals PrAlGe1-xSix with infrared spectroscopy and magneto-spectroscopy
RUI:利用红外光谱和磁光谱探讨外尔半金属 PrAlGe1-xSix 中磁性和相对论费米子之间的相互作用
  • 批准号:
    2323331
  • 财政年份:
    2023
  • 资助金额:
    $ 21万
  • 项目类别:
    Continuing Grant
RUI: Infrared Spectroscopy of the Martian Atmosphere
RUI:火星大气的红外光谱
  • 批准号:
    0805540
  • 财政年份:
    2008
  • 资助金额:
    $ 21万
  • 项目类别:
    Standard Grant
RUI: Infrared Spectroscopy of the Martian Atmosphere
RUI:火星大气的红外光谱
  • 批准号:
    0505765
  • 财政年份:
    2005
  • 资助金额:
    $ 21万
  • 项目类别:
    Standard Grant
RUI: Infrared Spectroscopy and Characterization of Comets
RUI:彗星的红外光谱和表征
  • 批准号:
    0407052
  • 财政年份:
    2004
  • 资助金额:
    $ 21万
  • 项目类别:
    Standard Grant
RUI: Collaborative Proposal: Visible and Near-Infrared Electronic Spectroscopy of Metal-Containing Diatomic Radicals from Intracavity Laser Absorption Techniques
RUI:合作提案:利用腔内激光吸收技术对含金属双原子自由基进行可见光和近红外电子光谱分析
  • 批准号:
    0213363
  • 财政年份:
    2002
  • 资助金额:
    $ 21万
  • 项目类别:
    Continuing Grant
RUI: Collaborative Proposal: Visible and Near-Infrared Electronic Spectroscopy of Metal-Containing Diatomic Radicals by Intracavity Laser Absorption Techniques
RUI:合作提案:通过腔内激光吸收技术对含金属双原子自由基进行可见光和近红外电子光谱分析
  • 批准号:
    0213356
  • 财政年份:
    2002
  • 资助金额:
    $ 21万
  • 项目类别:
    Continuing Grant
RUI: Crystal Growth Speedometry, Micro-Fourier Transform InfraRed (FTIR) Spectroscopy of Minerals
RUI:晶体生长速度测定、矿物的微傅立叶变换红外 (FTIR) 光谱
  • 批准号:
    0126285
  • 财政年份:
    2001
  • 资助金额:
    $ 21万
  • 项目类别:
    Continuing Grant
RUI: High Resolution Infrared Spectroscopy and Characterization of Comets
RUI:彗星的高分辨率红外光谱和表征
  • 批准号:
    0098411
  • 财政年份:
    2001
  • 资助金额:
    $ 21万
  • 项目类别:
    Continuing Grant
RUI: Infrared Spectroscopy as a Probe of Molecular Mechanismin the Hydrogenases
RUI:红外光谱作为氢化酶分子机制的探针
  • 批准号:
    9723828
  • 财政年份:
    1997
  • 资助金额:
    $ 21万
  • 项目类别:
    Standard Grant
RUI: Far-Infrared Spectroscopy of Oligonucleotides
RUI:寡核苷酸的远红外光谱
  • 批准号:
    9107105
  • 财政年份:
    1992
  • 资助金额:
    $ 21万
  • 项目类别:
    Standard Grant
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了