Stable Expression and Beta-adrenergic Receptor Subtype- Specific Regulation of the Cardiac L-type Ca2+ Channel

心脏 L 型 Ca2 通道的稳定表达和 β-肾上腺素能受体亚型特异性调节

• 批准号: 9509164
• 负责人: Atsuko Yatani
• 金额: $ 6万
• 依托单位: University of Cincinnati Main Campus
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-07-01 至 1997-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9509164&HistoricalAwards=false
• 关键词: Stable; Expression; Beta; adrenergic; Receptor

9509164 Yatani The voltage-dependent Ca2+ channel is a multi-subunit membrane- spanning protein that plays a critical role in the regulation of a variety of cell functions. The long-term goal of this research is to understand the physiological regulation of ion channels in the cardiovascular system. The identification of the mechanism underlying sympathetic modulation of the cardiac L-type Ca2+ channel is a key step in achieving this goal. Specific aims are: (1) To establish an useful expression system in which the molecular events which govern AR/L-type Ca2+ channel interactions can be deducted; (2) To define the relative contribution of -adrenergic subtypes ( 1AR and 2AR) to cardiac L-type Ca2+ channel regulation. The receptor subtype-specific regulation (both direct and indirect responses) will be compared in transfected cells; and (3) To investigate, at the molecular level, the regions of the AR which are critical for subtype-specific interactions with the Ca2+ channel. %%% The long-term goal of this research is to understand the physiological regulation of ion channels in the cardiovascular system. The research will take advantage of the cloning of the distinct beta-adrenergic receptor ( AR) subtypes and of the cardiac Ca2+ channel, and examine directly the regulatory mechanisms of AR on the Ca2+ channel at the molecular level. The results obtained from this research will serve as an initial data base for a more thorough study of the physiological regulation of ion channels. ***

电压依赖性Ca 2+通道是一种多亚基跨膜蛋白，在调节多种细胞功能中起关键作用。 本研究的长期目标是了解心血管系统中离子通道的生理调节。 识别心脏L型钙离子通道交感神经调制的机制是实现这一目标的关键步骤。 具体目标是：（1）建立一个有效的表达系统，在该系统中可以推导出AR/L型Ca ~（2+）通道相互作用的分子事件;（2）确定β-肾上腺素能亚型（1AR和2AR）对心脏L型Ca ~（2+）通道调节的相对贡献。 将在转染细胞中比较受体亚型特异性调节（直接和间接反应）;和（3）在分子水平上研究AR区域，该区域对于与Ca 2+通道的亚型特异性相互作用至关重要。 本研究的长期目标是了解心血管系统中离子通道的生理调节。 该研究将利用不同的β-肾上腺素能受体（AR）亚型和心脏Ca 2+通道的克隆，并直接研究β-肾上腺素能受体（AR）的调节机制。 AR在分子水平上对Ca 2+通道的作用。 本研究的结果将为更深入地研究离子通道的生理调节提供初步的数据基础。 ***