Acquisition of Equipment for Nucleic Acid and Protein Analysis

购置核酸和蛋白质分析设备

基本信息

  • 批准号:
    9512341
  • 负责人:
  • 金额:
    $ 15.97万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Standard Grant
  • 财政年份:
    1995
  • 资助国家:
    美国
  • 起止时间:
    1995-08-15 至 1997-07-31
  • 项目状态:
    已结题

项目摘要

Three items that would become components of our expanding molecular biology/biotechnology and environmental biology programs are solicited. These items are an automated DNA sequencer, a DNA synthesizer and a filmless autoradiography system. These tools for the synthesis of DNA and the analysis and quantitation of nucleic acids and proteins will extend research capabilities in numerous areas. A core of 12 research laboratories would directly benefit from the acquisition of this equipment. This core includes members from both the molecular biology/ biotechnology and the environmental biology programs in the department. A major Departmental priority has been to forge collaborations among these diverse research interests. It has become clear that the key to integrating these programs is molecular analysis. The molecular biologists routinely require nucleic acid sequence data for characterizing genes and verifying constructs. In addition these researchers must precisely quantify mRNA and protein levels to investigate gene regulation, and must synthesize oligonucleotides to manipulate specific DNA sequences. We also have an expanding environmental biology program that has strengths in conservation and population biology. The investigators of this program are relying increasingly on the power and resolution offered by molecular techniques. The problem encountered by population biologists is that the large sample sizes and extensive array of markers often required for these studies have restricted the wide scale use of laborious and expensive molecular techniques. The proposed equipment would be used jointly by both the molecular biology/ biotechnology and the environmental biology segments of our department. The equipment would serve as a focal point to foster collaborative and integrative research efforts. These projects range from basic research on gene regulation to studies of population dynamics and conservation genetics. The efficiency of these projects would be increased and all would be expanded and strengthened by the acquisition. The instrumentation would also have a significant impact on our environmental biology, biotechnology and molecular biology research training capabilities.
三个项目,将成为我们不断扩大的分子生物学/生物技术和环境生物学计划的组成部分征求。这些项目是一个自动DNA测序仪,DNA合成仪和无胶片放射自显影系统。这些用于DNA合成以及核酸和蛋白质的分析和定量的工具将扩展许多领域的研究能力。12个核心研究实验室将直接受益于购置这一设备。该核心包括来自分子生物学/生物技术和环境生物学计划的成员。一个主要部门的优先事项是建立这些不同的研究兴趣之间的合作。很明显,整合这些程序的关键是分子分析。 分子生物学家通常需要核酸序列数据来表征基因和验证构建体。此外,这些研究人员必须精确定量mRNA和蛋白质水平以研究基因调控,并且必须合成寡核苷酸以操纵特定的DNA序列。我们也有一个不断扩大的环境生物学计划,在保护和种群生物学的优势。该计划的研究人员越来越依赖于分子技术提供的能力和分辨率。种群生物学家遇到的问题是,这些研究通常需要大样本量和广泛的标记阵列,这限制了费力且昂贵的分子技术的广泛使用。 拟议的设备将由本部门的分子生物学/生物技术和环境生物学部门共同使用。这些设备将成为促进合作和综合研究努力的一个协调中心。这些项目的范围从基因调控的基础研究到种群动态和保护遗传学的研究。这些项目的效率将得到提高,所有这些项目都将通过收购得到扩大和加强。该仪器还将对我们的环境生物学、生物技术和分子生物学研究培训能力产生重大影响。

项目成果

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Richard Kesseli其他文献

Richard Kesseli的其他文献

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{{ truncateString('Richard Kesseli', 18)}}的其他基金

Genetic bases and ecological significance of plant-microbe interactions in the rhizospere
根际植物-微生物相互作用的遗传基础和生态意义
  • 批准号:
    0847691
  • 财政年份:
    2009
  • 资助金额:
    $ 15.97万
  • 项目类别:
    Continuing Grant
Dissertation Research: Genetic Diversity & Population Structure of Quercus ruba in Old Growth & Secondary Forests in Southern New England
论文研究:遗传多样性
  • 批准号:
    0073495
  • 财政年份:
    2000
  • 资助金额:
    $ 15.97万
  • 项目类别:
    Standard Grant
Genetic Basis for the Evolution of Dioecy in Silene
Silene雌雄异体进化的遗传基础
  • 批准号:
    9307823
  • 财政年份:
    1993
  • 资助金额:
    $ 15.97万
  • 项目类别:
    Continuing Grant

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