Acquisition of Instrumentation for Image Analysis in Biology

获取生物学图像分析仪器

• 批准号: 9512433
• 负责人: Lisa Baird
• 金额: $ 2.58万
• 依托单位: University of San Diego
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-08-01 至 1997-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9512433&HistoricalAwards=false
• 关键词: Acquisition; Instrumentation; Image; Analysis; Biology

This project proposes acquisition of instrumentation for image analysis and an inverted microscope to provide three research teams with the technological capability to rapidly and accurately analyze various organismal and tissue parameters. The instruments requested are the Leica Quantimet Q-500MC Image Analysis system with an Optronics Video Camera, and an Olympus IX-70 inverted microscope. The projects proposed involve both faculty research and research training primarily of undergraduates. The research experience is widely used as a teaching tool in our department. The research activities planned include a study of programmed cell death and cell cycle control in plants, study of muscle cell development and culture in fish, and a analysis of feeding behavior and movement in anostracans (fairy shrimp). Activities of these projects involve making large numbers of measurements from light and electron microscope data, analyzing labeling density, position and/or intensity in protein localization work, and assessing characteristics of organisms' three-dimensional morphology as it relates to movement. Currently some of the measurements required may be made by hand, other analyses are impossible without the computer capability. Acquisition of the image analysis system with archiving capability and the inverted microscope will enable us to rapidly and accurately obtain and analyze the data these studies require.

该项目建议购买图像分析仪器和倒置显微镜，为三个研究小组提供快速和准确分析各种生物和组织参数的技术能力。所需的仪器是带有光电摄像机的徕卡Quantimet Q-500MC图像分析系统和奥林巴斯IX-70倒置显微镜。建议的项目既涉及教员研究，也涉及主要针对本科生的研究培训。研究经验在我系被广泛用作教学工具。计划的研究活动包括植物细胞程序性死亡和细胞周期控制的研究，鱼类肌肉细胞发育和培养的研究，以及对龙舌兰(仙女虾)的摄食行为和运动的分析。这些项目的活动包括从光学和电子显微镜数据进行大量测量，分析蛋白质定位工作中的标记密度、位置和/或强度，以及评估与运动有关的生物体三维形态特征。目前，所需的一些测量可能是手工进行的，其他分析如果没有计算机的能力是不可能的。具有存档能力的图像分析系统和倒置显微镜的获取将使我们能够快速准确地获取和分析这些研究所需的数据。