Aquisition of Image Analysis Instrumentation for Environmental Microbiology, Cell Biology and Genetics Research and Teaching in an Undergraduate Institution

本科院校环境微生物学、细胞生物学和遗传学研究与教学图像分析仪器的购置

• 批准号: 0216180
• 负责人: David Huber
• 金额: $ 4.7万
• 依托单位: West Virginia State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-01 至 2005-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0216180&HistoricalAwards=false
• 关键词: Aquisition; Image; Analysis; Instrumentation; Environmental

A grant has been awarded to Dr. David H. Huber and Dr. Mark Chatfield atWest Virginia State College for the acquisition of an Image Analysis system. The digital image analysis applications that are required by many molecular biology techniques that involve DNA, RNA and protein imaging. This instrument will be used for environmental microbiology and cell biology research, as well as molecular biology instruction in several Biology Department classes. The environmental microbiology research involves a long-term study of microbial diversity and microbial metabolism in model bioprocessing reactors that are used for degrading organic matter. This research will demonstrate how the composition of a microbial community is linked to its overall metabolism. A second area of research will examine the molecular controls of plant cell division using model plant cell cultures.The goals of the environmental microbiology research are: 1) to examine the composition of the microbial communities in thermophilic anaerobic bioprocessing reactors, and 2) to determine the fate of antibiotic resistance genes that enter bioprocessing reactors.Bioprocessing reactors, also called digesters, transform organic waste into methane, a useful combustible gas, and fertilizer. The microbial interactions responsible for these chemical transformations in bioreactors are very complex and not well understood. Improvements in reactor biotechnology will be a practical benefit of this research. Theplant cell division research will improve our understanding of the molecular processes that govern plant growth. The analyzer will also be used for molecular biology training in several classes at West Virginia State College. The new instrument will significantly advance student training in molecular biology, microbiology and genetics.

大卫·H博士获得了一笔赠款。Huber和Mark Chatfield博士在西弗吉尼亚州立大学为图像分析系统的收购。 许多分子生物学技术所需的数字图像分析应用程序，涉及DNA，RNA和蛋白质成像。该仪器将用于环境微生物学和细胞生物学研究，以及生物系几门课程的分子生物学教学。 环境微生物学研究是在降解有机物的生物处理模型反应器中对微生物多样性和微生物代谢进行的长期研究。 这项研究将展示微生物群落的组成如何与其整体代谢相关联。 第二个研究领域将使用模式植物细胞培养物来研究植物细胞分裂的分子控制。环境微生物学研究的目标是：1）检查嗜热厌氧生物处理反应器中微生物群落的组成，以及2）确定进入生物处理反应器的抗生素抗性基因的命运。生物处理反应器，也称为消化器，将有机废物转化为甲烷，有用的可燃气体和肥料。 在生物反应器中负责这些化学转化的微生物相互作用非常复杂，并且还没有得到很好的理解。 反应堆生物技术的改进将是这项研究的实际好处。 植物细胞分裂的研究将提高我们对控制植物生长的分子过程的理解。 该分析仪还将用于西弗吉尼亚州立大学几个班级的分子生物学培训。 新仪器将大大推进分子生物学、微生物学和遗传学方面的学生培训。