Emersion Stress in Intertidal Seaweeds: Role of Active Oxygen

潮间带海藻的浮现应激：活性氧的作用

• 批准号: 9521341
• 负责人: Ian Davison
• 金额: $ 30.03万
• 依托单位: University of Maine
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-09-15 至 2000-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9521341&HistoricalAwards=false
• 关键词: Emersion; Stress; Intertidal; Seaweeds; Role

9521341 Davison The study will examine stress-tolerance in two major groups of perennial intertidal macroalgae, the red and brown seaweeds. The research will test the hypothesis that active oxygen is involved in emersion stress of intertidal seaweeds. Damage due to active oxygen will be determined in stress-tolerant and stress- susceptible species exposed to emersion stress by measuring the peroxidation of membrane lipids. Plants will be grown in laboratory culture under conditions that increase their ability to withstand emersion stress. If the research hypothesis is correct, increases in stress tolerance should be associated with increased levels of antioxidants and/or protective enzymes. The proposed research will also determine if intertidal seaweeds express specific stress-proteins in response to emersion stress. The results of the physiological studies will be used to design field experiments to measure the occurrence and importance of sublethal emersion stress in natural communities and to compare the allocation of resources to stress-tolerance in low and high shore species. This research represents one of the first attempts to understand the processes that confer stress tolerance in intertidal seaweeds and will provide valuable insights into the ecology and physiology of these plants. Information on the mechanism of stress tolerance will allow ecologists to assess the costs of stress tolerance and relate these to reproductive output, growth and competitive ability. ***

9521341 Davison 这项研究将检查两大类多年生潮间带大型藻类——红色海藻和棕色海藻——的应激耐受性。 该研究将验证活性氧与潮间带海藻的浮现应激有关的假设。 通过测量膜脂的过氧化，可以确定暴露于浮现胁迫的耐胁迫和胁迫敏感物种中由活性氧引起的损伤。 植物将在实验室培养条件下生长，以提高其抵抗芽苗胁迫的能力。 如果研究假设是正确的，那么压力耐受力的增加应该与抗氧化剂和/或保护酶水平的增加有关。 拟议的研究还将确定潮间带海藻是否表达特定的应激蛋白以应对浮现应激。 生理学研究的结果将用于设计现场实验，以测量自然群落中亚致死浮现应激的发生和重要性，并比较低岸和高岸物种的资源分配和应激耐受性。 这项研究是了解赋予潮间带海藻胁迫耐受性的过程的首批尝试之一，并将为这些植物的生态和生理学提供有价值的见解。 有关胁迫耐受机制的信息将使生态学家能够评估胁迫耐受的成本，并将其与繁殖产量、生长和竞争能力联系起来。 ***