A New Approach to Problems in Aggregate and Polyelectric Chemistry and Polypeptide Aggregate Topology

在有机动力学项目的资助下，Romsted教授将研究在表面活性剂表面上组装的多肽基质内部的化学环境。在这一努力中，一个分子探针（苯二氮鎓四氟硼酸盐）将作为组装的一部分合并。当被激活时，探针分子将分解并与其最近的邻居形成共价键。这样，探针分子附近的化学成分将被不可逆地捕获，并可以定量测量。这些实验的结果将首次提供多肽基质内反离子、醇和水合水的定量浓度。这一点很重要，因为组件内部的化学环境不同于散装溶液中的化学环境。由于这些空腔是反应发生的地方，因此更好地了解反应区域将有利于设计新药的研究人员，他们需要了解反应是如何发生的。这些类型的基质是生物反应组件的模型，如膜和胶束。生物系统组装分子阵列，形成反应容易发生的空腔。即预先安排反应物和电荷分布，以便于反应过程。因此，更彻底地了解这个项目所涉及的这些反应区，应该为科学家们提供制造更好的药物、建立人工反应区和更好地应对疾病的知识。本项目具有相当大的潜力和意义。

With funding from the Organic Dynamics Program, Professor Romsted will investigate the chemical environment inside a matrix of polypeptides which have been assembled on a surfactant surface. In this endeavor, a molecular probe(benzenediazonium tetrafluoroborate salt) will be coalesced as part of the assembly. When activated, the probe molecule will decompose to form covalent bonds with its nearest neighbors. In so doing, the chemical components near the probe molecule will be irreversibly trapped and can be measured quantitatively. Results of these experiments should provide, for the first time, quantitative concentrations of counterions, alcohols and waters of hydration inside polypeptide matrices. This is significant in that the chemical environment inside an assembly differs from that in the bulk solution. Since these cavities are where reactions occur, a better understanding of reactive region should benefit researchers who design new drugs and need to understand how reactions occur. These types of matrices are models for biological reaction assemblies such as membranes and micelles. Biological systems assemble molecular arrays to form cavities where reactions occur with ease. That is , the reactants and the charge distributions are prearranged so as to facilitate the reaction processes. Thus, a more thorough understanding of these reaction zones, which this project addresses, should provide scientists with the knowledge to make better drugs, to build artificial reaction zones and better cope with diseases. This proposed project has considerable potential and significance.