SBIR Phase II: Genetic Fingerprinting and Registry for Psittacine Birds

SBIR 第二阶段：鹦鹉遗传指纹和登记

• 批准号: 9529743
• 负责人: John Patton
• 金额: $ 26.47万
• 依托单位: LGL ECOLOGICAL RESEARCH ASSOCIATES, INC
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-07-15 至 1998-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9529743&HistoricalAwards=false
• 关键词: SBIR; Phase; II; Genetic; Fingerprinting

*** 9529743 Patton This Small Business Innovation Research Phase II project will develop a prototype automated microsatellite genotyping system for commercial applications. In Phase I of this project, nine microsatellite loci were isolated from hyacinth macaws. These loci were used for DNA fingerprinting of individual hyacinth macaws and pedigrees. These loci were also found to work in six other macaw species. In Phase II of this project, the system will be expanded to include six species from the other major groups of psittacine birds: cockatoos, Old World Parrots, and New World Parrots. Using new molecular cloning methods, developed in Phase I, 6-10 microsatellite loci in each of the six new species will isolated and characterized. Protocols will be developed for multiplex PCR(TM) reactions and analysis of multiple loci in a single lane of an electrophoretic gel on an automated DNA sequencer. Genotype data will be linked directly from the laboratory system to a computerized database. It is anticipated that at the end of Phase II, a complete, efficient, and cost-effective system will be available for producing a multilocus genotype suitable for individual identity and pedigree analysis for most species of psittacines. There is a large existing market for genotyping services in parrots and many other species including livestock, pets, laboratory animals, and wild populations. This market is expected to grow substantially for parrots as the Wild Bird Conservation Act is enforced and positive identity of legally-owned birds is required. As modern, cost-effective systems, such as that to be developed in this research, become available it is anticipated that the market for genotyping other species will grow rapidly. ***

*** 9529743 Patton这个小企业创新研究二期项目将开发一个用于商业应用的原型自动化微卫星基因分型系统。本项目一期从风信子金刚鹦鹉中分离出9个微卫星位点。这些位点被用于单个风信子金刚鹦鹉和家系的DNA指纹图谱。这些基因座也被发现在其他六种金刚鹦鹉中起作用。在该项目的第二期，该系统将扩大到包括其他主要鹦鹉种群的六个物种：凤头鹦鹉、旧大陆鹦鹉和新世界鹦鹉。利用第一阶段开发的新分子克隆方法，将从6个新物种的每个物种中分离出6-10个微卫星位点并进行鉴定。将开发用于多重PCR（TM）反应的方案，并在自动DNA测序仪上的电泳凝胶单行道上分析多个位点。基因型数据将直接从实验室系统连接到计算机化数据库。预计在第二阶段结束时，将有一个完整、高效和经济的系统可用于生产适合大多数鹦鹉物种的个体身份和谱系分析的多位点基因型。对鹦鹉和许多其他物种（包括家畜、宠物、实验动物和野生种群）的基因分型服务有很大的现有市场。随着《野生鸟类保护法》的实施和合法拥有的鸟类的身份要求，预计鹦鹉市场将大幅增长。随着现代的、具有成本效益的系统（如本研究中开发的系统）的出现，预计对其他物种进行基因分型的市场将迅速增长。＊＊＊