Enhancement of Undergraduate Instruction in the Biochemistryand Analysis of Proteins

该项目通过开发分子细胞生物学和蛋白质生物化学技术的实验课程来加强大学的本科教育，并获得色谱和电泳设备来支持这门课程。本课程通过对酪氨酸酶进行净化和分析，为学生提供蛋白质层析、电泳以及蛋白质物理和酶性质分析的实践经验，然后将数据以标准期刊格式的论文形式写出来。学生在色谱柱的制备和操作、蛋白质的电泳分析、分光光度法、酶测定和动力学分析以及研究实验室中使用的分析方法和决策过程方面获得了丰富的经验。这种经历为学生进入细胞和分子生物学的研究生学习提供了强大的背景。此外，新设备还支持分子生物学、微生物学和免疫学以及细胞生物学等现有课程的实验室。最后，该色谱设备使学生能够开发和执行没有该设备就无法考虑的本科研究项目。

This project enhances undergraduate education at the university through the development of a laboratory course in techniques in molecular cell biology and protein biochemistry, and obtains chromatography and electrophoresis equipment to support this course. The course provides students with hands-on experience in protein chromatography, electrophoresis, and analysis of the physical and enzymatic properties of a protein by purifying and analyzing the enzyme tyrosinase in a multiweek integrated experiment and then writing up the data as a paper in the standard journal format. Students gain extensive experience in the preparation and operation of chromatography columns, electrophoretic analysis of proteins, spectrophotometry, enzyme assays, and kinetic analysis and in the analytical methods and decision-making process that are used in research laboratories. This experience provides students with a strong background for entering graduate studies in cell and molecular biology. In addition, the new equipment supports labs in existing courses in molecular biology, microbiology and immunology, and cell biology. Finally, this chromatography equipment enables students to develop and perform undergraduate research projects that could not be considered without this equipment.