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RNA editing and DYW-type PPR proteins in the moss Physcomitrella patens

苔藓小立碗藓中的 RNA 编辑和 DYW 型 PPR 蛋白

基本信息

批准号：
105061384
负责人：
Professor Dr. Volker Knoop
金额：
--
依托单位：
Institut für Zelluläre und Molekulare Botanik (IZMB)
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2008
资助国家：
德国
起止时间：
2007-12-31 至 2012-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/105061384?language=en
关键词：
RNA editing DYW type PPR

项目摘要

The RNA-binding PPR (Pentatricopeptide Repeat) proteins are known to be involved in organellar RNA maturation events. Their wide expansion into large gene families with more than 400 members in angiosperms and their frequent targeting into mitochondria and chloroplasts had raised speculations on their involvement, as specificity factors, also in the abundant RNA editing events in the two plant organelles. This has recently received strong support from functional genetic analyses in Ambidopsis. Exploring a unique carboxyterminal extension, which is highly conserved in a plant-specific sub-clade of PPR proteins, the DYW domain, we have recently found that the plant-specific DYW proteins are also present in jungermanniid, but not in marchantiid liverworts. This is fully congruent with observations that RNA editing is present in the former but not in the latter liverwort class. We also found both, frequent RNA editing and DYW-type PPR protein diversity, in the isolated, basal liverwort genus Haplomitrium. We have now explored mitochondrial RNA editing diversity in the transcriptome of the experimentally amenable model moss Physcomitrella patens. In line with the above observations, we find a very limited number of RNA editing sites in Physcomitrella (11), again in congruence with a low diversity of DYW-proteins (10) now deducible from the completed moss genomic sequence. We here suggest to make use of Physcomitrella as a model system to functionally investigate DYW proteins and RNA editing, employing the novel RNA immuno co-precipitation (RIP-CHIP) methodology.

已知RNA结合的PPR （Pentatricopeptide Repeat）蛋白参与细胞器RNA成熟事件。它们在被子植物中广泛扩展到拥有400多个成员的大基因家族中，并且它们经常靶向线粒体和叶绿体，这引发了人们对它们作为特异性因子参与两种植物细胞器中丰富的RNA编辑事件的猜测。这一观点最近得到了Ambidopsis功能性遗传分析的有力支持。在探索PPR蛋白的植物特异性亚分支DYW结构域的独特羧基末端延伸时，我们最近发现植物特异性DYW蛋白也存在于jungermanniid中，但不存在于marchantiid liverworts中。这与RNA编辑存在于前者而不存在于后者的观察结果完全一致。我们还在分离的底苔属Haplomitrium中发现了频繁的RNA编辑和dyw型PPR蛋白多样性。我们现在已经探索了线粒体RNA编辑的多样性，在实验可适应的模式苔藓小壶藓的转录组。根据上述观察结果，我们发现小立壶菌的RNA编辑位点数量非常有限（11），这再次与现在从完整的苔藓基因组序列中推断出的低多样性dyw蛋白（10）相一致。我们建议利用新型RNA免疫共沉淀（RIP-CHIP）方法，以小壶菌为模型系统，对DYW蛋白和RNA编辑进行功能研究。