Collaborative Research: RESEARCH-PGR: Development of epigenetic editing for crop improvement
合作研究:RESEARCH-PGR:用于作物改良的表观遗传编辑的开发
基本信息
- 批准号:2331438
- 负责人:
- 金额:$ 30.74万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Standard Grant
- 财政年份:2024
- 资助国家:美国
- 起止时间:2024-05-01 至 2028-04-30
- 项目状态:未结题
- 来源:
- 关键词:
项目摘要
Genome editing tools have revolutionized biology. Scientists are using these tools to connect genes to phenotypes, generate novel phenotypic variation, and for diverse crop improvement applications. This project expands the possibilities for editing in crop plants to include a method for editing gene expression, so called “epigenetic editing”. Epigenetics is a broad term used to describe mechanisms that change gene expression without directly changing the DNA. Epigenetic variability can have profound impacts on an organism’s phenotype, and many important agronomic traits are influenced by gene expression. Broader impacts of the project include the wide dissemination of the method for the improvement of crop plants and other species, plus the training of students via integration with a long running and successful undergraduate internship program. Epigenetic crop improvement strategies will complement existing biotechnology and breeding strategies and may offer opportunities to help maintain crop yields in the face of climate change. This project will directly train the next generation of scientists to use these tools through an effective Research Experiences for Undergraduates (REU) program. All resources generated in this project will be made available for use.This project will expand the possibilities for editing in crop plants to include epigenetic editing. It was recently demonstrated that it is possible to affect gene expression through targeted DNA methylation at MeSWEET10a in the important crop, cassava. MeSWEET10a is not normally expressed in leaf tissue but is ectopically induced by a bacterial pathogen using a transcription activator-like (TAL) effector. De novo methylation of the MeSWEET10a promoter blocked TAL binding and led to decreased disease symptoms. Despite these encouraging results, epigenetic editing is still an immature technology. The goal of this project is to fill specific knowledge gaps related to establishment, maintenance, and inheritance of epigenetic edits and in so doing, lower the entry point for other researchers to adopt this powerful technology. Specifically, aim 1 of this proposal is to develop and distribute epigenetic editing tools in other crop species. The work will leverage and adapt the technologies known to work in cassava to accomplish similar disease outcomes in rice and tomato. Aim 2 will expand on the applications of epigenetic editing in crops. This includes the ability to target multiple loci simultaneously, “fine tune” gene expression and tissue specific editing. Aim 3 is dedicated to characterizing stability and heritability of de novo epialleles. If successful, other research projects and applications in biotechnology will benefit. This work will also provide additional jumping off points for fundamental work on epigenetics in non-model plant systems. Further, an ability to directly edit the epigenome will empower the larger field of epigenetics.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
基因组编辑工具彻底改变了生物学。科学家们正在使用这些工具将基因与表型联系起来,产生新的表型变异,并用于各种作物改良应用。该项目扩大了作物编辑的可能性,包括编辑基因表达的方法,即所谓的“表观遗传编辑”。表观遗传学是一个广泛的术语,用于描述改变基因表达而不直接改变DNA的机制。表观遗传变异对生物体的表型有着深远的影响,许多重要的农艺性状都受到基因表达的影响。该项目更广泛的影响包括广泛传播作物和其他物种改良方法,以及通过与长期运行和成功的本科生实习计划相结合来培训学生。表观遗传作物改良战略将补充现有的生物技术和育种战略,并可能提供机会,帮助在气候变化的情况下保持作物产量。该项目将通过有效的本科生研究经验(REU)计划直接培训下一代科学家使用这些工具。该项目产生的所有资源将可供使用。该项目将扩大作物植物编辑的可能性,包括表观遗传编辑。最近证明,在重要作物木薯中,有可能通过靶向MeSWEET10a的DNA甲基化来影响基因表达。MeSWEET10a通常不在叶组织中表达,但由细菌病原体使用转录激活因子样(TAL)效应子异位诱导。MeSWEET10a启动子的从头甲基化阻断TAL结合并导致疾病症状减轻。尽管有这些令人鼓舞的结果,表观遗传编辑仍然是一项不成熟的技术。该项目的目标是填补与表观遗传编辑的建立,维护和继承相关的特定知识空白,并在此过程中降低其他研究人员采用这种强大技术的切入点。具体而言,该提案的目标1是在其他作物物种中开发和分发表观遗传编辑工具。这项工作将利用和调整已知在木薯中工作的技术,以在水稻和番茄中实现类似的疾病结果。目标2将扩大表观遗传编辑在作物中的应用。这包括同时靶向多个基因座、“微调”基因表达和组织特异性编辑的能力。目的3致力于表征从头表观等位基因的稳定性和遗传性。如果成功,其他生物技术研究项目和应用将受益。这项工作也将为非模式植物系统中表观遗传学的基础工作提供额外的起点。此外,直接编辑表观基因组的能力将赋予更大的表观遗传学领域以力量。该奖项反映了NSF的法定使命,并被认为值得通过使用基金会的智力价值和更广泛的影响审查标准进行评估来支持。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Bing Yang其他文献
Study protocol for Vascular Access outcome measure for function: a vaLidation study In hemoDialysis (VALID)
血管通路功能结果测量的研究方案:血液透析验证研究(有效)
- DOI:
- 发表时间:
2022 - 期刊:
- 影响因子:2.3
- 作者:
A. Viecelli;A. Teixeira;A. Valks;R. Baer;R. Cherian;P. Cippà;J. Craig;R. Desilva;A. Jaure;David W. Johnson;C. Kiriwandeniya;P. Kopperschmidt;Wen;Timmy Lee;C. Lok;K. Madhan;Alistair R. Mallard;Veronica Oliver;K. Polkinghorne;R. Quinn;D. Reidlinger;M. Roberts;B. Sautenet;L. Hooi;Rob Smith;M. Snoeijs;J. Tordoir;T. Vachharajani;R. Vanholder;L. Vergara;M. Wilkie;Bing Yang;T. Yuo;L. Zou;Carmel M. Hawley;Laura Alyssa Sunil V. Neil Katrina Yeoungjee Michael Mag Robison Welch Badve Boudville Campbell Cho Collins;Laura Robison;Alyssa Welch;S. Badve;N. Boudville;K. Campbell;Yeoungjee Cho;Michael Collins;M. Fahim;M. Jardine;Dianne Du Toit;Michelle Mayne;K. Stevenson;Rachel James;Q. Vu;K. Allen;Leanne Glancy;Jijo Kumbikkal;Sharan Burton;L. Gordon;Kyle McCarthy;Cathy Forrester;Sally Lima;O. Bourgault;Claire Drouault;F. Teasdale;L. Jiun;Jamian Abidin;Cheng Jin Kiang;Lee Soon Leng;Yuana Mohd Yusoff;Adriana Ciochina;M. V. van Loon;Ronald Ophelders;M. Vleugels;P. Ferrari;M. Brodeur;D. Giunzioni;C. Molfese;C. Blackwell;Louese Dunn;Laura Gillis;Barry E. Gray;S. Jenkins - 通讯作者:
S. Jenkins
Using Chlorine Atoms to Fine-Tune the Intermolecular Packing and Energy Levels of Efficient Nonfullerene Acceptors
使用氯原子微调高效非富勒烯受体的分子间堆积和能级
- DOI:
10.1021/acsaem.9b01667 - 发表时间:
2019-10 - 期刊:
- 影响因子:6.4
- 作者:
Hanjian Lai;Hui Chen;Yue Shen;Meijing Wang;Pengjie Chao;Weicheng Xie;Jianfei Qu;Bing Yang;Feng He - 通讯作者:
Feng He
Improving the removal of fine particles by heterogeneous condensation during WFGD processes
在 WFGD 过程中通过非均相冷凝改善细颗粒的去除
- DOI:
10.1016/j.fuproc.2016.01.033 - 发表时间:
2016-05 - 期刊:
- 影响因子:0
- 作者:
Lin-jun Yang;Jin-pei Yan;Guang-xin Hong;Bing Yang - 通讯作者:
Bing Yang
The Density Functional Theory Investigation on the Structural, Relative Stable and Electronic Properties of Bimetallic PbnSbn (n = 2–12) Clusters
双金属PbnSbn(n = 2−12)团簇结构、相对稳定性和电子性质的密度泛函理论研究
- DOI:
10.1007/s10876-018-14 - 发表时间:
2018 - 期刊:
- 影响因子:2.8
- 作者:
Gaofeng Li;Xiumin Chen;Hongwei Yang;Baoqiang Xu;Bing Yang;Dachun Liu - 通讯作者:
Dachun Liu
The Fast Readout Unit for General Control and Data Acquisition in Heavy-ion Experiments
用于重离子实验中通用控制和数据采集的快速读出装置
- DOI:
10.1109/tns.2022.3232118 - 发表时间:
2022 - 期刊:
- 影响因子:1.8
- 作者:
Haibo Yang;Honghui Zhang;Baiyang Zhu;Bing Yang;Qi Zhang;Jinxiang Wang;Liangrong Li;Chengxin Zhao - 通讯作者:
Chengxin Zhao
Bing Yang的其他文献
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{{ truncateString('Bing Yang', 18)}}的其他基金
Collaborative Research: RESEARCH-PGR: Genome-wide quest for non-host resistance mechanisms in plants
合作研究:RESEARCH-PGR:全基因组探索植物非宿主抗性机制
- 批准号:
2224204 - 财政年份:2023
- 资助金额:
$ 30.74万 - 项目类别:
Standard Grant
Collaborative Research: Mechanisms of pollen-specific phospholipases in maize haploid production
合作研究:花粉特异性磷脂酶在玉米单倍体生产中的机制
- 批准号:
2222158 - 财政年份:2022
- 资助金额:
$ 30.74万 - 项目类别:
Standard Grant
RESEARCH-PGR: SECRETome Project: Systematic Evaluation of CellulaR ExporT from plant cells
RESEARCH-PGR:SECRETome 项目:植物细胞 CellulaR ExportT 的系统评估
- 批准号:
1936492 - 财政年份:2019
- 资助金额:
$ 30.74万 - 项目类别:
Continuing Grant
RESEARCH-PGR: SECRETome Project: Systematic Evaluation of CellulaR ExporT from plant cells
RESEARCH-PGR:SECRETome 项目:植物细胞 CellulaR ExportT 的系统评估
- 批准号:
1810468 - 财政年份:2017
- 资助金额:
$ 30.74万 - 项目类别:
Continuing Grant
Collaborative Research: The role of host nutrient carriers in pathogen susceptibility
合作研究:宿主营养载体在病原体易感性中的作用
- 批准号:
1258103 - 财政年份:2013
- 资助金额:
$ 30.74万 - 项目类别:
Standard Grant
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