Japan JSPS Program: Use of Pyrolysis Gas Chromatography Mass Spectrometry for Assessing the State of Preservation of Proteinaceous Materials

日本 JSPS 计划：使用热解气相色谱质谱法评估蛋白质材料的保存状态

• 批准号: 9600262
• 负责人: Mary Becker
• 金额: $ 0.3万
• 依托单位: Fellowships
• 依托单位国家: 美国
• 项目类别: Fellowship Award
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-09-01 至 1997-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9600262&HistoricalAwards=false
• 关键词: Japan; JSPS; Program; Use; Pyrolysis

9600262 Becker This award supports a 12 month Japan Society for the Promotion of Science (JSPS) Postdoctoral Fellowship for Dr. Mary Becker at the Tokyo National Research Institute of Cultural Properties, Tokyo, Japan. Dr. Becker will work with Dr. Chie Sano, senior researcher in the Conservation Science Department ast the Institute, on a research project entitled, "The Use of Pyrolysis Gas Chromatography Mass Spectrometry for Assessing the State of the Preservation of Proteinaceous Materials". The proposed research will develop analytical techniques sensitive to chemical alterations prior to their manifestation as physical changes in museum objects. A gas chromatograph (GC) interfaced with a mass spectrometry (MS) and combined with an initial pyrolysis (Py) step will be used to reveal the concentrations and number of pyrolysis products of Nylon 6,6 change as a function of heat treatment. Since soluble fragments with a distribution of molecular weights are formed as silk deteriorates, it is anticipated that this technique (Py-GC-MS) will be useful in monitoring the chemical alterations as a function of aging. Ascertaining the complex nature of natural degradation and that which occurs during pyrolysis, the research will examine if changes in the fragmentation patterns of naturally degraded proteins in silk can indicate the state of preservation. A systematic study of aged silks could not only supply information on the state of preservation of silk but will also determine the usefulness of this technique as a strategy for routine analysis of proteinaceous materials found in cultural property objects. ***

小行星9600262 该奖项支持玛丽贝克尔博士在日本东京国立文化财产研究所获得为期12个月的日本科学促进协会（JSPS）博士后奖学金。 贝克尔博士将与该研究所保护科学系的高级研究员千惠佐野博士一起进行一项题为“利用热解气相色谱质谱法评估蛋白质材料的保存状况”的研究项目。 拟议的研究将开发对化学变化敏感的分析技术，然后再将其表现为博物馆物品的物理变化。 将使用气相色谱（GC）与质谱（MS）接口并结合初始热解（Py）步骤，以揭示尼龙6，6热解产物的浓度和数量随热处理的变化。 由于分子量分布的可溶性片段形成的丝绸恶化，预计这种技术（Py-GC-MS）将是有用的，在监测作为老化的函数的化学变化。 确定自然降解的复杂性质和热解过程中发生的复杂性质，研究将检查丝绸中自然降解蛋白质的碎片模式的变化是否可以指示保存状态。 对老化丝绸的系统研究不仅可以提供有关丝绸保存状况的信息，而且还将确定这种技术作为文化财产物品中发现的蛋白质材料的常规分析策略的有用性。 ***