Japan JSPS Program: Use of Pyrolysis Gas Chromatography Mass Spectrometry for Assessing the State of Preservation of Proteinaceous Materials

9600262 Becker该奖项为日本东京国立文化财产研究所的Mary Becker博士提供为期12个月的日本科学促进会（JSPS）博士后奖学金。贝克尔博士将与研究所保存科学部高级研究员Chie Sano博士合作，开展一项名为“使用热解气相色谱质谱法评估蛋白质材料保存状况”的研究项目。拟议的研究将开发对化学变化敏感的分析技术，在它们表现为博物馆物品的物理变化之前。采用气相色谱（GC）与质谱（MS）相结合，结合初始热解（Py）步骤，揭示尼龙6,6热解产物的浓度和数量随热处理的变化。由于具有分子量分布的可溶性碎片是在丝绸变质时形成的，因此预计该技术（Py-GC-MS）将用于监测作为老化功能的化学变化。确定自然降解和热解过程中发生的复杂性质，该研究将检验蚕丝中自然降解蛋白质破碎模式的变化是否可以表明保存状态。对古丝绸的系统研究不仅可以提供有关丝绸保存状况的信息，而且还将确定该技术作为对文物中发现的蛋白质物质进行常规分析的策略的实用性。＊＊＊

9600262 Becker This award supports a 12 month Japan Society for the Promotion of Science (JSPS) Postdoctoral Fellowship for Dr. Mary Becker at the Tokyo National Research Institute of Cultural Properties, Tokyo, Japan. Dr. Becker will work with Dr. Chie Sano, senior researcher in the Conservation Science Department ast the Institute, on a research project entitled, "The Use of Pyrolysis Gas Chromatography Mass Spectrometry for Assessing the State of the Preservation of Proteinaceous Materials". The proposed research will develop analytical techniques sensitive to chemical alterations prior to their manifestation as physical changes in museum objects. A gas chromatograph (GC) interfaced with a mass spectrometry (MS) and combined with an initial pyrolysis (Py) step will be used to reveal the concentrations and number of pyrolysis products of Nylon 6,6 change as a function of heat treatment. Since soluble fragments with a distribution of molecular weights are formed as silk deteriorates, it is anticipated that this technique (Py-GC-MS) will be useful in monitoring the chemical alterations as a function of aging. Ascertaining the complex nature of natural degradation and that which occurs during pyrolysis, the research will examine if changes in the fragmentation patterns of naturally degraded proteins in silk can indicate the state of preservation. A systematic study of aged silks could not only supply information on the state of preservation of silk but will also determine the usefulness of this technique as a strategy for routine analysis of proteinaceous materials found in cultural property objects. ***