Glycoallcaloids of Temperate and Tropical Solanum Species: Patterns of Occurrence and Ecologocial Relevance

温带和热带茄属植物的糖苷类化合物：发生模式和生态相关性

• 批准号: 9601505
• 负责人: Martin Cipollini
• 金额: $ 3.38万
• 依托单位: Berry College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-09-01 至 1998-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9601505&HistoricalAwards=false
• 关键词: Glycoallcaloids; Temperate; Tropical; Solanum; Species

In this proposal, funds are being requested for the acquisition, setup, and maintenance of a number of pieces of equipment used for the analysis of primary and secondary chemicals found in plants. Specific items include a high-pressure liquid chromatography HPLC) unit, accessories to a low-pressure liquid column (LC) chromatography apparatus, a centrifuge, a refrigerating/heating water bath, a ductless fume hood, a an ultrafreezer, an analytical balance, a pH meter, a hot plate, a vortex mixer, a Wiley mill, and a set of micropipetters. These instruments will be used principally in a research program designed to quantify a number of related secondary compounds (glycoalkaloids) in fruits of Solanum species and to isolate these compounds for use in bioassays with birds, mammals, microbes, and insects. Such studies are critical for understanding the role of secondary compounds of ripe fleshy fruits in mediating interactions among plants and the organisms that consume their fruits. Of particular interest is the apparent evolutionary paradox of "toxic" fruits and the relative effects of fruit toxins on organisms with contrasting effects upon seed viability and seed dispersal. This research was initiated at the University of Florida under NSF grant DEB-9207920 (Douglas J. Levey, PI) and is being continued by the co-PI of this grant and the project director of the current proposal, Dr. Martin L. Cipollini, with financial support for materials and supplies from Berry College. Dr. Cipollini began a tenure-track faculty position at Berry College in August 1995. Because of a historical lack of academic research instrumentation at this college and because of the necessity of leaving research equipment at the University of Florida upon his leaving, it has been very difficult for Dr. Cipollini to resume the laboratory-based components of his research. Acquisition of the above items will allow the resumption of this research and will allow this research to expand i n other directions in the future. Of particular concern is the desire to involve undergraduates in Dr. Cipollini's research program. Berry College has a guaranteed student work program, which gives faculty researchers virtually unlimited access to qualified undergraduate research assistants, generally two or three students per semester. With the instrumentation to conduct the research, undergraduates will be able to be fully involved in all aspects of this research program. Students will have the opportunity of acting as research assistants, of being involved in directed studies (independent research for credit), and of conducting honors-level research. Moreover, use of a number of these pieces of equipment will be shared with at least three other faculty researchers at Berry College, Drs. Chris Mowry, Bill Davin, and Laura Fielden, who share an unimproved lab (Cook 302) with Dr. Cipollini. Availability of these instruments to these researchers will likewise enhance their research programs and their ability to support undergraduate research and education at Berry College.

在该提案中，要求提供资金，用于购置、安装和维护一些设备，用于分析工厂中发现的一级和二级化学品。具体项目包括高压液相色谱（HPLC）单元、低压液相柱（LC）色谱装置的附件、离心机、制冷/加热水浴、无管通风柜、超冷冻机、分析天平、pH计、热板、涡旋混合器、Wiley研磨机和一套微量移液器。这些仪器将主要用于一项研究计划，旨在量化茄属物种果实中的一些相关二级化合物（配糖生物碱），并分离这些化合物用于鸟类、哺乳动物、微生物和昆虫的生物测定。这些研究对于理解成熟肉质果实的次级化合物在介导植物和食用其果实的生物体之间的相互作用中的作用至关重要。特别令人感兴趣的是明显的进化悖论的“有毒”水果和水果毒素对生物体的相对影响与种子活力和种子传播的对比效果。 这项研究是在美国国家科学基金会资助DEB-9207920（道格拉斯J. Levey，PI）下在佛罗里达大学发起的，并由该资助的共同PI和当前提案的项目主任Martin L. Cipollini，与财政支持的材料和用品从贝瑞学院。Cipollini博士于1995年8月开始在贝里学院担任终身教职。由于历史上缺乏学术研究仪器在这所大学，并因为必须离开后，在佛罗里达大学的研究设备，这一直是非常困难的博士。收购上述项目将使这项研究得以恢复，并将使这项研究在未来向其他方向扩展。 特别令人关注的是让本科生参与Cipollini博士的研究计划的愿望。贝瑞学院有一个有保证的学生工作计划，这使得教师研究人员几乎可以无限制地获得合格的本科生研究助理，通常每学期两到三名学生。有了进行研究的仪器，本科生将能够充分参与这项研究计划的各个方面。学生将有机会担任研究助理，参与定向研究（独立研究学分），并进行荣誉级研究。此外，这些设备的使用将与贝瑞学院的至少三名其他教师研究人员共享，他们是克里斯·莫里博士、比尔·达文博士和劳拉·菲尔登博士，他们与奇波里尼博士共享一个未经改进的实验室（库克302）。这些仪器的可用性，这些研究人员也将提高他们的研究计划和他们的能力，以支持本科研究和教育在贝里学院。