Automated DNA Sequencer

在过去的十年中，DNA测序已经成为生物和生物医学研究的主要基石之一。 尽管普林斯顿大学校园内有40多个研究小组必须将DNA测序作为他们正在进行的研究计划的一部分，但这些小组目前使用的程序效率低下，耗时，而且越来越过时。为此，分子生物学系正在申请资金购买自动DNA测序仪器。该仪器包括以下项目： ABI Prism自动DNA测序仪，型号377-01，包括安装和现场培训、数据收集软件、Macintosh power Mac、两（2）个凝胶盒、顶部和底部凝胶灌注混合物（1套）和用户手册 测序软件模块，包括测序分析软件、手册和化学指南、2个36孔Sharkstooth梳和测序安装套件 快速测序或高分辨率GeneScan玻璃板/间隔物试剂盒，包括36 cm W-T-R玻璃板（2个正面/2个背面）和36 cm间隔物 HP 1200 c打印机 GenePCR系统9600，包括用户手册、快速参考手册、说明视频、耗材启动套件 Auto Assembler，5件装，用户安装，包括Factura和Auto Assembler应用软件，教程，用户和参考手册，在线动画软件，参考鼠标垫 Sequence Navigator 5-pak DNA和蛋白质序列比较软件，包括Factura和Sequence Navigator应用软件、教程、用户和参考手册 该仪器将安置在现有的部门服务设施内，并由该设施内经过专门培训的人员进行操作。 仪器的日常操作将在Syn/Seq机构总监S.金Syn/Seq设施中的自动DNA测序仪器将用于实现四个主要目标。 首先是提供有效和快速的DNA测序。 第二个是提供DNA测序服务，这将有利于整个普林斯顿生物和生物医学研究界。 这不仅包括分子生物学系的所有成员，还包括校园内其他几个部门的生物和生物医学研究科学家。 这些部门包括化学，生态学和进化生物学，物理学和材料科学。 第三是大幅减少科学家和技术人员现在必须投入到DNA测序中的时间和精力。 这里的目的是使我们的研究社区的成员，以指导他们的努力沿着其他潜在的更富有成效的，技术上困难和/或创新的调查路线。 第四，DNA测序设施也将纳入我们的本科生培训计划。

During the past ten years DNA sequencing has become one of the major cornerstones of biological and biomedical research. Although more than 40 research groups on the Princeton campus must sequence DNA as part of their ongoing research programs, the procedures currently used by these groups are inefficient, time consuming, and increasingly antiquated. For this reason, the Department of Molecular Biology is requesting funds for the purchase of automated DNA sequencing instrumentation. This instrumentation includes the following items: ABI Prism Automated DNA Sequencer, Model 377-01, including installation and on-site training, data collection software, Macintosh power Mac, two (2) Gel Cassettes, top and bottom gel pouring mixtures (1 set), and user manual Sequencing software module, including sequencing analysis software, Manual and Chemistry Guide, two (2) 36 Well Sharkstooth combs, and the sequencing installation kit Fast sequencing or high resolution GeneScan glass plates/ spacers kit, including 36cm W-T-R Glass plates (2 front/2 back), and 36cm Spacers HP 1200c Printer GeneAmp PCR System 9600, including user manual, Quick Reference Manual, Instructional Video, Consumables Start-up kit Auto Assembler, 5-pak, user installed, includes Factura and Auto Assembler Application software, tutorial, User and Reference Manuals, On-line animation software, reference mouse pad Sequence Navigator 5-pak DNA and Protein Sequence Comparison Software, including Factura and Sequence Navigator application software, tutorial, User and Reference Manuals This instrumentation will be housed in an existing Departmental Service Facility, and it will be operated by specially trained personnel in this facility. The daily operation of the instrumentation will be under the direct supervision of the Syn/Seq Facility Director, Mr. S. Kyin. The automated DNA sequencing instrumentation in the Syn/Seq Facility will be used to achieve four major goals. The first is to provide efficien t and rapid DNA sequencing. The second is to provide a DNA sequencing service that will benefit the entire Princeton biological and biomedical research community. This will include not only all members of the Molecular Biology Department, but also biological and biomedical research scientists in several other Departments on campus. These departments include Chemistry, Ecology and Evolutionary Biology, Physics and Material Sciences. The third is to substantially reduce the time and effort that scientists and technicians must now devote to DNA sequencing. The aim here is to enable members of our research community to direct their efforts along other potentially more productive, technically difficult and/or innovative lines of investigation. Fourth, the DNA Sequencing Facility will also be incorporated in our undergraduate training programs.