Automated DNA Sequencer

自动DNA测序仪

• 批准号: 9601840
• 负责人: Paul Schedl
• 金额: $ 10.3万
• 依托单位: Princeton University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-07-15 至 1998-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9601840&HistoricalAwards=false
• 关键词: Automated; DNA; Sequencer

During the past ten years DNA sequencing has become one of the major cornerstones of biological and biomedical research. Although more than 40 research groups on the Princeton campus must sequence DNA as part of their ongoing research programs, the procedures currently used by these groups are inefficient, time consuming, and increasingly antiquated. For this reason, the Department of Molecular Biology is requesting funds for the purchase of automated DNA sequencing instrumentation. This instrumentation includes the following items: ABI Prism Automated DNA Sequencer, Model 377-01, including installation and on-site training, data collection software, Macintosh power Mac, two (2) Gel Cassettes, top and bottom gel pouring mixtures (1 set), and user manual Sequencing software module, including sequencing analysis software, Manual and Chemistry Guide, two (2) 36 Well Sharkstooth combs, and the sequencing installation kit Fast sequencing or high resolution GeneScan glass plates/ spacers kit, including 36cm W-T-R Glass plates (2 front/2 back), and 36cm Spacers HP 1200c Printer GeneAmp PCR System 9600, including user manual, Quick Reference Manual, Instructional Video, Consumables Start-up kit Auto Assembler, 5-pak, user installed, includes Factura and Auto Assembler Application software, tutorial, User and Reference Manuals, On-line animation software, reference mouse pad Sequence Navigator 5-pak DNA and Protein Sequence Comparison Software, including Factura and Sequence Navigator application software, tutorial, User and Reference Manuals This instrumentation will be housed in an existing Departmental Service Facility, and it will be operated by specially trained personnel in this facility. The daily operation of the instrumentation will be under the direct supervision of the Syn/Seq Facility Director, Mr. S. Kyin. The automated DNA sequencing instrumentation in the Syn/Seq Facility will be used to achieve four major goals. The first is to provide efficien t and rapid DNA sequencing. The second is to provide a DNA sequencing service that will benefit the entire Princeton biological and biomedical research community. This will include not only all members of the Molecular Biology Department, but also biological and biomedical research scientists in several other Departments on campus. These departments include Chemistry, Ecology and Evolutionary Biology, Physics and Material Sciences. The third is to substantially reduce the time and effort that scientists and technicians must now devote to DNA sequencing. The aim here is to enable members of our research community to direct their efforts along other potentially more productive, technically difficult and/or innovative lines of investigation. Fourth, the DNA Sequencing Facility will also be incorporated in our undergraduate training programs.

在过去的十年中，DNA测序已成为生物学和生物医学研究的主要基石之一。尽管普林斯顿大学校园里有40多个研究小组必须对DNA进行测序，作为他们正在进行的研究项目的一部分，但这些小组目前使用的程序效率低下，耗时，而且越来越过时。出于这个原因，分子生物系正在申请资金用于购买自动DNA测序仪器。该仪器包括以下项目：ABI Prism自动DNA测序仪，型号377-01，包括安装和现场培训，数据采集软件，Macintosh power Mac，两(2)个凝胶盒，顶部和底部凝胶灌胶混合物（1套），和用户手册测序软件模块，包括测序分析软件，手册和化学指南，两个(2)36 Well Sharkstooth梳子，以及测序安装套件快速测序或高分辨率GeneScan玻璃板/间隔器套件。包括36cm W-T-R玻璃板（2个前/2个后）和36cm Spacers HP 1200c Printer GeneAmp PCR System 9600，包括用户手册，快速参考手册，教学视频，消费品启动套件Auto Assembler， 5包，用户安装，包括Factura和Auto Assembler应用软件，教程，用户和参考手册，在线动画软件，参考鼠标垫Sequence Navigator 5包DNA和蛋白质序列比较软件，包括Factura和Sequence Navigator应用软件、教程、用户和参考手册。该仪器将被安置在现有的部门服务设施中，并将由该设施中受过专门培训的人员操作。仪器的日常操作将由Syn/Seq设备总监S. Kyin先生直接监督。Syn/Seq设施中的自动化DNA测序仪器将用于实现四个主要目标。首先是提供高效和快速的DNA测序。第二个目标是提供DNA测序服务，这将使整个普林斯顿生物和生物医学研究界受益。这不仅包括分子生物系的所有成员，还包括校园其他几个系的生物和生物医学研究科学家。这些系包括化学、生态和进化生物学、物理和材料科学。第三是大大减少科学家和技术人员现在必须投入DNA测序的时间和精力。这里的目的是使我们的研究界的成员能够指导他们的努力沿着其他可能更有成效，技术上困难和/或创新的调查线。第四，DNA测序设施也将纳入我们的本科培训计划。