Modular Approach to Biotechnology Laboratory Instruction Based on a Novel Green-Fluorescent Protein

基于新型绿色荧光蛋白的生物技术实验室教学模块化方法

• 批准号: 9602356
• 负责人: William Ward
• 金额: $ 35万
• 依托单位: Rutgers University New Brunswick
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-07-15 至 1999-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9602356&HistoricalAwards=false
• 关键词: Modular; Approach; Biotechnology; Laboratory; Instruction

Modular Approach to Biotechnology Laboratory Instruction Based on a Novel Green-Fluorescent Protein This project is developing laboratory modules in biotechnology for secondary school and undergraduate level students and their teachers, based on a unique protein, the green-fluorescent protein (GFP), which serves as an easily visualized reporter for gene expression and all steps in protein purification. Detection requires only a long-wave UV lamp, making the system adaptable to low-budget curricula. Because the protein is so easily visualized, it enhances the students' understanding of molecular biology and biochemistry making each step in the laboratory exercise an exciting experience. Initial concepts for GFP-based laboratory modules were developed at Rutgers in 1989 and have been successfully "field tested" for five years. Nine other institutions including one other major research university, one four-year college, two county colleges, three high schools, one major biochemical supply corporation, and one national laboratory are joining Rutgers in an informal consortium to advance the GFP-based laboratory module concept. Originally isolated from a bioluminescent jellyfish. GFP can now be cloned into other organisms. Thus. the gene and its protein are available for widespread use. The project is developing GFP-based laboratory modules geared for the advanced secondary school and junior college levels. The modular "kit" approach to teaching biotechnology techniques allows both protein purification and recombinant DNA techniques to be transferred to all appropriate settings with only minor modifications. This GFP-based modular approach provides major changes in the way technicians are trained and can be projected on a national scale to be widely applied in the biotechnology community.

基于新型绿色荧光蛋白的生物技术实验教学模块该项目正在为中学生和本科水平的学生及其教师开发生物技术实验模块，该模块基于一种独特的蛋白质-绿色荧光蛋白(GFP)，它可以作为基因表达和蛋白质纯化所有步骤的可视化报告程序。检测只需要一个长波紫外线灯，使该系统适用于低预算的课程。由于蛋白质很容易被可视化，它增强了学生对分子生物学和生物化学的理解，使实验室中的每一步练习都是一次令人兴奋的经历。基于GFP的实验室模块的最初概念是1989年在罗格斯大学开发的，并已成功地进行了五年的“现场测试”。包括另一所主要研究型大学、一所四年制学院、两所县级学院、三所高中、一家大型生化供应公司和一家国家实验室在内的另外九家机构将加入罗格斯大学的一个非正式联盟，以推进基于GFP的实验室模块概念。最初是从一种生物发光水母中分离出来的。绿色荧光蛋白现在可以克隆到其他生物体中。因此。这种基因及其蛋白质可以广泛使用。该项目正在开发基于GFP的实验室模块，面向高级中学和大专水平。教授生物技术技术的模块化“工具包”方法允许蛋白质纯化和重组DNA技术只需稍加修改就可以转移到所有适当的环境中。这种基于GFP的模块化方法对技术人员的培训方式进行了重大改变，并可在全国范围内推广应用于生物技术社区。