Modular Approach to Biotechnology Laboratory Instruction Based on a Novel Green-Fluorescent Protein

基于新型绿色荧光蛋白的生物技术实验教学模块化方法本项目基于绿色荧光蛋白（GFP）这一独特的蛋白质，作为基因表达和蛋白质纯化的所有步骤的易于可视化的报告者，开发面向中学部学生和教师的生物技术实验模块。检测只需要一个长波紫外线灯，使系统适应低预算的课程。因为这种蛋白质很容易可视化，它增强了学生对分子生物学和生物化学的理解，使实验室练习的每一步都是令人兴奋的体验。基于gfp的实验室模块的最初概念于1989年在罗格斯大学开发出来，并已成功进行了五年的“现场测试”。其他九所机构，包括一所主要研究型大学、一所四年制学院、两所县学院、三所高中、一家主要生化供应公司和一家国家实验室，正在加入罗格斯大学的非正式联盟，以推进基于gfp的实验室模块概念。最初是从生物发光水母中分离出来的。绿色荧光蛋白现在可以克隆到其他生物体中。因此。该基因及其蛋白质可广泛使用。该项目正在开发面向高级中学和大专水平的基于gfp的实验室模块。教学生物技术的模块化“工具包”方法允许将蛋白质纯化和重组DNA技术转移到所有适当的环境中，只需要进行微小的修改。这种基于gfp的模块化方法使技术人员的培训方式发生了重大变化，并且可以在全国范围内进行预测，以便在生物技术界得到广泛应用。

Modular Approach to Biotechnology Laboratory Instruction Based on a Novel Green-Fluorescent Protein This project is developing laboratory modules in biotechnology for secondary school and undergraduate level students and their teachers, based on a unique protein, the green-fluorescent protein (GFP), which serves as an easily visualized reporter for gene expression and all steps in protein purification. Detection requires only a long-wave UV lamp, making the system adaptable to low-budget curricula. Because the protein is so easily visualized, it enhances the students' understanding of molecular biology and biochemistry making each step in the laboratory exercise an exciting experience. Initial concepts for GFP-based laboratory modules were developed at Rutgers in 1989 and have been successfully "field tested" for five years. Nine other institutions including one other major research university, one four-year college, two county colleges, three high schools, one major biochemical supply corporation, and one national laboratory are joining Rutgers in an informal consortium to advance the GFP-based laboratory module concept. Originally isolated from a bioluminescent jellyfish. GFP can now be cloned into other organisms. Thus. the gene and its protein are available for widespread use. The project is developing GFP-based laboratory modules geared for the advanced secondary school and junior college levels. The modular "kit" approach to teaching biotechnology techniques allows both protein purification and recombinant DNA techniques to be transferred to all appropriate settings with only minor modifications. This GFP-based modular approach provides major changes in the way technicians are trained and can be projected on a national scale to be widely applied in the biotechnology community.