Renovation of Protein Engineering Facilities

蛋白质工程设施改造

• 批准号: 9602617
• 负责人: Charles Craik
• 金额: $ 21.19万
• 依托单位: University of California-San Francisco
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-10-15 至 1998-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9602617&HistoricalAwards=false
• 关键词: Renovation; Protein; Engineering; Facilities

Funding from the Academic Research Infrastructure Program will enable the University of California at San Francisco to renovate existing research space dedicated to protein engineering studies. The primary focus of the Laboratory has been on understanding and applying structure-function relationships in proteins with a particular concentration on proteases and their natural inhibitors using genetic and biochemical methods. Protein engineering technologies are used to create wild-type and variant proteins in reagent quantities that are then analyzed both biochemically and biophysically to study substrate specificity, catalytic mechanism and structure. Located on the ninth floor of the UCSF Medical Sciences Building, the facilities provide a unique window on the principles and practice of protein engineering and its potential application to biotechnology. Research space, constructed 40 years ago that has been used for various significant scientific contributions in protein engineering since 1985, will be renovated. These facilities include four rooms: Molecular Biochemistry Laboratory, a Fermentation and Sterilization Room, a Cold Room, and a Computer Modeling Room. The lack of items such as no sinks, drains, vacuum and natural gas will be provided, a fume hood will be installed, and repairs to the electrical and refrigeration systems will be made. The Program is extremely productive and actively recruits high school, undergraduate, and minority students who aspire to become scientists interested in interdisciplinary research. Students experience many aspects of biotechnology from computer modeling to hands-on protein engineering to structure determination. Not only will this project enhance collaborations, but it will promote the development of new macromolecular tools needed to conduct state- of-the-art biotechnology research.

来自学术研究基础设施计划的资金将使加州大学旧金山分校弗朗西斯科能够改造现有的致力于蛋白质工程研究的研究空间。 该实验室的主要重点是理解和应用蛋白质的结构-功能关系，特别是蛋白酶及其天然抑制剂，使用遗传和生物化学方法。 蛋白质工程技术用于以试剂量产生野生型和变体蛋白质，然后对其进行生化和生物药理学分析以研究底物特异性，催化机制和结构。该设施位于UCSF医学科学大楼的九楼，为蛋白质工程的原理和实践及其在生物技术中的潜在应用提供了一个独特的窗口。 40年前建造的研究空间自1985年以来一直用于蛋白质工程的各种重大科学贡献，将被翻新。 这些设施包括四个房间：分子生物化学实验室，发酵和灭菌室，冷藏室和计算机建模室。 将提供诸如没有水槽、排水管、真空和天然气等物品，安装通风橱，并修理电气和制冷系统。 该计划是非常富有成效的，并积极招募高中，本科和少数民族学生谁渴望成为科学家感兴趣的跨学科研究。 学生体验生物技术的许多方面，从计算机建模到动手蛋白质工程到结构测定。 该项目不仅将加强合作，还将促进进行最先进生物技术研究所需的新大分子工具的开发。