Towards Commercial Protein Production through Co-Engineering Marine Microorganisms and Feedstocks for Precision Fermentation

通过联合设计海洋微生物和精密发酵原料实现商业蛋白质生产

• 批准号: 10086903
• 金额: $ 29.56万
• 依托单位: BRAVELYCULTURED LTD
• 依托单位国家: 英国
• 项目类别: Collaborative R&D
• 财政年份: 2024
• 资助国家: 英国
• 起止时间: 2024 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=10086903
• 关键词: Towards; Commercial; Protein; Production; through

We are redesigning precision fermentation to enable truly-scalable animal-free food production.Unsustainable agriculture and livestock industry are among the key drivers of GHG emissions, land use change and water use. To fight against environmental degradation, the food industry has been looking at innovative technologies to produce animal-free proteins. Precision fermentation allows production of biological compounds in microorganisms. Through this method, animal proteins can be produced in yeast and fungi and utilised for human consumption. However, precision fermentation is a technology developed over 50 years ago and optimised for the production of pharmaceutical high-value compounds like insulin. Without fundamental redesigning it cannot solve the key challenges that the current food industry is facing.At BravelyCultured we are a team of PhD scientists and food supply-chain sustainability experts and we are selecting for novel marine microorganisms and seaweed-based feedstocks for precision fermentation. Our goal is to develop the technology which will decouple our food-supply chains from agriculture and shift it towards utilisation of the most plentiful resource on the planet -- the ocean.The core objective of this project is to co-optimize our seaweed-based feedstocks and the most promising yeast and fungal strains to enhance cellulase enzyme secretion capacity. Cellulase finds extensive use in wine, coffee, juice, paper, and textile industries. Our aim is to provide a cost-effective and sustainably produced source of cellulase (high yielding cellulase secreting microorganism growing on optimal seaweed based feedstock), creating a readily commercialisable outcome.Our novel marine microorganisms naturally produce cellulase when cultivated on seaweed feedstocks. To further enhance cellulase yields, we will employ directed evolution and random mutagenesis techniques. Collaborating with Multus Biotechnology, experts in feedstock development, we will optimise our seaweed-based feedstocks, significantly improving the economics of precision fermentation.In the long term, we envision applying synthetic biology approaches to engineer our optimised strains for animal protein production on sustainable feedstocks, forming the cornerstone of our commercialisation strategy. Through this grant, we are eager to unlock the vast potential of precision fermentation and pave the way for a more sustainable and ethical food future.

我们正在重新设计精确发酵，以实现真正无动物的粮食生产。不可自我的农业和牲畜行业是温室气体排放，土地使用变化和用水的关键动力。为了与环境退化作斗争，食品行业一直在研究生产无动物蛋白质的创新技术。精度发酵允许生产微生物中的生物化合物。通过这种方法，动物蛋白可以在酵母和真菌中生产，并用于人类食用。但是，精确发酵是一项技术，它是50年前开发的，并针对制药高价值化合物（如胰岛素）进行了优化。没有基本的重新设计，它将无法解决当前食品行业面临的关键挑战。在勇敢的文化中，我们是一支由博士学位科学家和食品供应链可持续性专家组成的团队，我们正在选择新颖的海洋微生物和基于海藻的原料以进行精确发酵。我们的目标是开发将我们的食品供应链从农业中解脱出来的技术，并将其转变为利用地球上最丰富的资源 - 海洋。该项目的核心目的是将基于海藻的原料和最有前途的酵母菌和酵母菌菌株配合使用，以增强蜂窝酶Enzyme eNzymeemecyme的能力。纤维素酶发现在葡萄酒，咖啡，果汁，纸和纺织工业中广泛使用。我们的目的是提供一种具有成本效益且可持续产生的纤维素酶来源（高产纤维素酶在基于最佳海藻的原料上生长的微生物分泌），从而创造出易于商业性的结果。当在海藻原料上种植时，我们的新型海洋微生物自然会产生纤维素酶。为了进一步增强纤维素酶的产量，我们将采用定向进化和随机诱变技术。我们将与多种生物技术，原料开发专家合作，我们将优化基于海藻的原料，大大改善精确发酵的经济学。在长期来看，我们设想将合成生物学方法应用于构成可持续原料的动物蛋白质生产的优化菌株，以使我们的商业化策略构成我们的商业化策略。通过这笔赠款，我们渴望释放精确发酵的巨大潜力，并为更加可持续和道德的粮食未来铺平道路。