Replacement of Induced Mutant Resource Facilities

位于缅因州巴尔港的杰克逊实验室(TJL)是ARI资金的接受者，用于取代现有的诱导突变资源(IMR)研究空间。IMR成立于1992年，是一个国家转基因、靶向突变和化学诱导突变小鼠的冷冻保存和分布中心。目前在不同设施中进行的IMR服务包括：健康状况未知的小鼠的进口、胚胎的冷冻保存以及需要不寻常的健康保障或繁育模式的小鼠的维护和近亲繁殖。然而，随着这些进程的快速增长，这些进程的整合对于国际生物研究的成功至关重要，这对在所有领域进行生物学研究越来越重要，在现有条件下是不可能实现的。这些设施的配置严重限制了质量控制功能，现在需要单独的、重复的胚胎移植设施和动物群体。缺乏足够的空间阻碍了IRM接受菌株作为冷冻胚胎或配子保存，以及开发和实施新的动物再生方法。这个项目是TJL为IMR建造永久设施的更大努力的一部分。具体地说，NSF资金将用于取代SPF和冷冻保存设施的研究空间。整合这些功能将允许TJL只接受超低温保存的菌株，增加IMR可以保存的菌株数量，同时保持存储库的成本可控。作为实验室研究和教育发展战略计划的重要组成部分，替代结构将促进发展生物学、分子遗传学、神经遗传学和行为学项目的招聘工作。研究将得到加强，以及高级培训计划，研究人员可以进入IMR的诱导突变模型的聚宝盆。该项目将确保这一科学储备的可行性。

The Jackson Laboratory (TJL), located in Bar Harbor, Maine, is the recipient of ARI funds for the replacement of existing research space for the Induced Mutant Resource (IMR). Established in 1992, the IMR is a national center for the cryopreservation and distribution of transgenic, targeted mutant, and chemically induced mutant mice. Current services of the IMR that occur in separate facilities include: importation of mice of unknown health status, cryopreservation of embryos, and the maintenence and inbreeding of mice which require unusual health safeguards or breeding paradigms. However with rapid growth, the integration of these processes are imperative to the success of the IMR which is increasingly important to the conduct of biological research in all areas, and cannot be achieved under existing conditions. The configuration of the facilities presents serious limitations to quality control functions that now require separate, duplicative embryo transfer facilities and animal colonies. The lack of adequate space hinders the IRM from accepting strains for preservation as cryopreserved embryos or gametes, and from developing and implementing new methods for rederiving animals. This project is part of a larger effort of TJL to construct a permanent facility for the IMR. Specifically, NSF funds will be used to replace research space for the SPF and Cryopreservation facilities. Consolidating these functions will allow the TJL to accept strains for cryopreservation only, increasing the number of strains the IMR can preserve, while keeping costs of the repository manageable. As an important component of the Laboratory's strategic plans for research and educational growth, the replacement structure will facilitate recruitment efforts in building programs in developmental biology, molecular genetics and neurogenetics and behavior. Research will be enhanced as well as advanced training programs where research personnel have access to the IMR's cornucopia of induced mutant models. This project will ensure the viability of this scientific reserve.