Acquisition of Bio-Imaging Instrumentation

收购生物成像仪器

• 批准号: 9603072
• 负责人: John Perona
• 金额: $ 7.7万
• 依托单位: University of California-Santa Barbara
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-05-01 至 1999-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9603072&HistoricalAwards=false
• 关键词: Acquisition; Bio; Imaging; Instrumentation

The proposal requests funds to purchase several instruments for image analysis: an integrated phosphor-imager and direct fluorescence/chemifluorescence system, a film recorder, and a MacIntosh computer and dedicated system laser printer. The three instruments will provide critical image analysis capabilities presently unavailable to Chemistry and Biology Department graduate students, post-doctoral fellows and faculty. Major research projects that will benefit from these instruments are as follows: (1) Definition of structural and energetic parameters for specific DNA binding and cleavage by a restriction endonuclease- a model system for studying enzyme-DNA complexes; (2) Definition of catalytic and recognition mechanisms of bacterial DNA methyltransferases; (3) Mechanisms of iron acquisition by marine micro-organisms; (4) Expression and characterization of double-stranded RNA dependent enzymes under interferon regulation; (5) Definition of the molecular mechanisms involved in Ras function; (6) Structure- function relationships of nuceic acid polymerases and microtubule-binding proteins studied by crystallographic and biochemical approaches; (7) Structure-function analysis of the acetylcholine transporter of synaptic vesicles.

该提案要求提供资金购买几种图像分析仪器：集成的荧光成像仪和直接荧光/化学荧光系统、胶片记录仪以及麦金托什计算机和专用系统激光打印机。 这三台仪器将提供目前化学和生物系研究生、博士后研究员和教师无法获得的关键图像分析能力。 将受益于这些仪器的主要研究项目如下：⑴确定限制性内切核酸酶特异性DNA结合和切割的结构和能量参数-研究酶-DNA复合物的模型系统; ⑵确定细菌DNA甲基转移酶的催化和识别机制; ⑶海洋微生物获得铁的机制;（4）干扰素调节下双链RNA依赖性酶的表达和表征;（5）Ras功能相关分子机制的定义;（6）通过晶体学和生物化学方法研究核酸聚合酶和微管结合蛋白的结构-功能关系;（7）突触囊泡乙酰胆碱转运蛋白的结构-功能分析。