Molecular and Genetic Analysis of Developmental and Hormonal Signals Regulating Tomato Fruit Ripening

调节番茄果实成熟的发育和激素信号的分子和遗传分析

• 批准号: 9604115
• 负责人: James Giovannoni
• 金额: $ 37.63万
• 依托单位: Texas A&M Research Foundation
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-02-01 至 2000-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9604115&HistoricalAwards=false
• 关键词: Molecular; Genetic; Analysis; Developmental; Hormonal

9604115 Giovannoni Dr. Giovannoni treats ripening of the tomato fruit as a developmental program - in contrast to most of other analyses in which the tomato fruit is studied as an example of plant senescence. Some ethylene-insensitive tomato mutants, indeed, indicate that the senescence-specific hormone ethylene can be modulated in a developmental fashion. His group has isolated from tomato genes homologous to components of the ethylene signal transduction pathway detected in other plants. In combination with his mutant collection and the detection of fruit-specific transcripts that do not respond to ethylene but are under other developmental control, the project develops. It includes the characterization and transgenic manipulation of the CTR1 ethylene signal transduction modulator as one aspect. Secondly, constitutive ethylene responding mutants are characterized physiologically and with respect to the expression of developmentally-regulated transcripts. Finally, non-ethylene responsive, developmentally induced transcripts will be functionally characterized. The work is expected to provide an integrative view of different gene expression programs regulating fruit ripening in general.

9604115 Giovannoni Giovannoni博士将番茄果实的成熟视为一个发育过程，这与大多数其他分析中将番茄果实作为植物衰老的一个例子进行研究不同。 事实上，一些乙烯不敏感的番茄突变体表明，衰老特异性激素乙烯可以在发育的方式进行调制。 他的研究小组已经从番茄中分离出与在其他植物中检测到的乙烯信号转导途径组分同源的基因。 结合他的突变体收集和水果特异性转录物的检测，这些转录物对乙烯没有反应，但受到其他发育控制，该项目得以发展。 一方面包括CTR 1乙烯信号转导调节剂的表征和转基因操作。 其次，组成型乙烯响应突变体的特征在于生理和发育调控的转录本的表达。 最后，将对非乙烯响应的、发育诱导的转录本进行功能表征。 这项工作有望提供一个综合的观点，不同的基因表达程序调节水果成熟一般。