Molecular Control of Flower Development

花发育的分子控制

• 批准号: 9604507
• 负责人: Jack Okamuro
• 金额: $ 21万
• 依托单位: University of California-Santa Cruz
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-02-15 至 2000-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9604507&HistoricalAwards=false
• 关键词: Molecular; Control; Flower; Development

OKAMURO 9604507 Arabidopsis flower development is governed by an evolutionarily conserved network of genes that control the establishment and maintenance of flower meristem identity, floral organ development, and gametogenesis. However, little is known about how these genes function together at the cell and molecular levels. The APETALA2 (AP2) gene plays a central role in controlling flower development in Arabidopsis, in part, by controlling the temporal and spatial expression pattern of another floral homeotic gene AGAMOUS (AG). The AP2 protein represents the first of what is now known to be an important new family of plant DNA binding proteins including EREBPs, AINTEGUMENTA, and TINY. The goal of the experiments in this project is to understand how AP2 controls AG gene expression at the molecular level by analyzing its DNA binding activity in vitro and to identify proteins that may physically interact with and function together with AP2 during flower development. Specific goals include: (1) identifying sequences within the AP2 polypeptide that are required for AP2 DNA binding activity in vitro, (2) identifying sequences within the AP2 polypeptide that are required for AP2 function in viva, (3) determining how AP2 gene activity is regulated at the posttranslational level, (4) determining whether AP2 forms multimers with itself or with other AP2 domain containing proteins in viva, and (5) identifying proteins that form stable physical interactions with AP2 using gel filtration chromatography. The elucidation of AP2 function will ultimately provide new insight into the control of plant growth and development at the molecular level.

OKAMURO 9604507 拟南芥花的发育受到进化上保守的基因网络的控制，这些基因控制着花分生组织特性的建立和维持、花器官的发育和配子发生。然而，人们对这些基因如何在细胞和分子水平上共同发挥作用知之甚少。 APETALA2 (AP2) 基因在控制拟南芥花发育中发挥着核心作用，部分是通过控制另一个花同源异型基因 AGAMOUS (AG) 的时间和空间表达模式。 AP2 蛋白代表了目前已知的重要新植物 DNA 结合蛋白家族中的第一个蛋白，其中包括 EREBP、AINTEGUMENTA 和 TINY。该项目实验的目的是通过分析其体外 DNA 结合活性，了解 AP2 如何在分子水平上控制 AG 基因表达，并鉴定在花发育过程中可能与 AP2 物理相互作用并共同发挥作用的蛋白质。具体目标包括：(1)鉴定AP2多肽内AP2 DNA体外结合活性所需的序列，(2)鉴定AP2多肽内AP2体内功能所需的序列，(3)确定AP2基因活性如何在翻译后水平调节，(4)确定AP2是否在体内与自身或与其他含有AP2结构域的蛋白质形成多聚体，以及 (5)使用凝胶过滤色谱法鉴定与AP2形成稳定物理相互作用的蛋白质。 AP2功能的阐明最终将为在分子水平上控制植物生长和发育提供新的见解。