Rapid Kinetics Analysis Center

快速动力学分析中心

• 批准号: 9604675
• 负责人: Gloria Ferreira
• 金额: $ 5万
• 依托单位: University of South Florida
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-03-15 至 2000-02-29
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9604675&HistoricalAwards=false
• 关键词: Rapid; Kinetics; Analysis; Center

Rapid kinetic analyses are essential for biochemical research Important information on the mechanism and regulation of enzymes, the mechanism of interaction between proteins and nucleic acids, and on the binding of signal transduction molecules (i.e., ligands) to receptor proteins can be derived from the use of stopped-flow spectrophotometry. Most enzymes have turnover numbers between 1 and 106 s-1, which implies that the rate constants for the reactions between intermediates will be usually greater than 1 s-1 and that each step will take less than 1 second to occur. In this proposal we will detail the use of rapid stopped-flow spectrophotometry for the investigation of specific biochemical reactions and the development of a Rapid Kinetics Analysis Center that will facilitate its use and be capable of upgrading the instrumentation.

快速动力学分析是生物化学研究必不可少的关于酶的机制和调节、蛋白质和核酸之间相互作用的机制以及信号转导分子（即，配体）与受体蛋白质的结合可以通过使用停流分光光度法得到。大多数酶的周转数在1到106 s-1之间，这意味着中间体之间反应的速率常数通常大于1 s-1，并且每个步骤发生的时间不到1秒。在本提案中，我们将详细介绍使用快速停流分光光度法进行特定生化反应的调查，并开发一个快速动力学分析中心，以促进其使用，并能够升级仪器。