An Optical Biosensor Instrument for Analysis of Biomolecular Interactions in Molecular and Cellular Biology

用于分析分子和细胞生物学中生物分子相互作用的光学生物传感器仪器

• 批准号: 9604805
• 负责人: Stephen Kowalczykowski
• 金额: $ 6.79万
• 依托单位: University of California-Davis
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-07-01 至 1998-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9604805&HistoricalAwards=false
• 关键词: Optical; Biosensor; Instrument; Analysis; Biomolecular

This is a proposal to purchase an optical biosensor instrument for research in molecular and cellular biology. The proposed instrument uses a cuvette-based design to measure, in real-time, the interaction between different molecules. The technology is based on an optical biosensor which detects macromolecular binding due to changes in refractive index at the boundary of the biosensor surface. This instrument permits real-time, analysis of molecular interactions. A variety of molecular interactions will be studied. These include both kinetic and thermodynamic analyses of hormone-receptor, enzyme-substrate, receptor- cell, DNA-DNA, DNA-protein, and protein-protein interactions. These interactions are central to processes in molecular and cellular biology such as cell motility, genetic recombination, protein transport, RNA transcription, and signal transduction. These interactions can now be studied in real time over a wide dynamic range of size and concentration, and without the need for radioisotopic, or other, labeling. Application of this new technology will greatly facilitate fundamental research on cellular function and will contribute to the training of future biological scientists.

这是一个购买光学生物传感器的建议 用于分子和细胞生物学研究仪器。 该仪器采用基于比色皿的设计， 实时测量不同的 分子。 该技术基于一种光学生物传感器， 检测由于折射率变化而引起的大分子结合 在生物传感器表面的边界处的折射率。这 仪器允许实时分析分子 交互.各种分子间的相互作用 研究了这些包括动力学和热力学 酶-受体、酶-底物、受体- 细胞、DNA-DNA、DNA-蛋白质和蛋白质-蛋白质相互作用。 这些相互作用是分子生物学过程的核心。 和细胞生物学，如细胞运动，遗传 重组，蛋白质转运，RNA转录，和 信号转导这些相互作用现在可以在 真实的时间在一个宽的动态范围的大小和 浓度，而不需要放射性同位素，或 其他，标签。 应用 的 这项新技术 将 大大 促进细胞功能的基础研究， 为培养未来的生物科学家做出贡献。