Enhancing Biosensor Surface Functionalization for new applications in Drug Development & Production

增强生物传感器表面功能化以实现药物开发的新应用

• 批准号: 10090247
• 金额: $ 6.54万
• 依托单位: HEXAGONFAB LIMITED
• 依托单位国家: 英国
• 项目类别: Collaborative R&D
• 财政年份: 2024
• 资助国家: 英国
• 起止时间: 2024 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=10090247
• 关键词: Enhancing; Biosensor; Surface; Functionalization; new

Antibody therapeutics are a class of drugs with synthetic antibodies as the active ingredient. They offer highly targeted and effective treatments for a wide range of diseases. The analysis of antibodies during drug development presents significant challenges due to their complexity. First, assessing their binding affinity and specificity necessitates intricate methods to ensure optimal therapeutic properties. Second, the production of antibodies demands sophisticated production and monitoring, making the entire drug development process time-consuming and resource intensive. Antibodies are predominantly analysed in central labs, which can lead to delays in obtaining results and hinder real-time decision-making. Implementing decentralized analysis closer to the point of need, will offer faster turnaround times and expedite decision-making.HexagonFab's instrument, Bolt, is specifically designed for point-of-need analysis of antibodies. Currently, Bolt is limited to working with purified solutions. This means that any sample must undergo a process to remove unwanted substances or impurities prior to analysis. Many applications in various fields necessitate compatibility with crude media, i.e. complex samples containing mixtures of different biomolecules without undergoing extensive purification. This compatibility allows for more versatile application of the instrument in diverse settings.HexagonFab is broadening its sensor portfolio by introducing a new generation of sensors capable of measuring crude samples. These sensors feature an electrically sensitive thin film coated with biomolecules; a process known as surface functionalization. The surface functionalization employed is a unique protein/graphene composite material, offering optimal electrical conductivity, while ensuring low non-specific binding signals.To ensure successful product launch, the optimal production and storage conditions for achieving the ideal surface functionalization must be identified. This includes testing of various production methods followed by precise chemical analysis to evaluate the properties of the surface functionalization. In collaboration with NPL, this project aims to evaluate the influence of different production processes on the surface functionalization. By identifying the most effective process conditions, HexagonFab can enhance the performance of its sensors and ensure the successful development of advanced technologies.Understanding and optimizing the surface coating process will ensure that the sensors exhibit the required sensitivity and specificity. With their ability to measure crude samples, these sensors can directly interface with crude media, allowing for more accurate and efficient antibody analysis without the need for extensive purification, ultimately saving time and resources.

抗体疗法是一类具有合成抗体作为活性成分的药物。他们为多种疾病提供了高度针对性和有效的治疗方法。药物开发过程中抗体的分析由于其复杂性带来了重大挑战。首先，评估其结合亲和力和特异性需要复杂的方法来确保最佳的治疗特性。其次，抗体的生产需要复杂的生产和监测，使整个药物开发过程耗时和资源大量。抗体主要在中央实验室中进行分析，这可能导致延迟获得结果并阻碍实时决策。实施分散的分析更接近需求，将提供更快的周转时间并加快决策。Hexagonfab的仪器Bolt是专门设计用于抗体需要分析的。目前，螺栓仅限于使用纯化解决方案。这意味着任何样本都必须在分析之前经历一个过程以去除不良物质或杂质。在各个领域的许多应用都需要与粗介质的兼容，即包含不同生物分子混合物的复杂样品，而无需进行广泛的纯化。这种兼容性允许该仪器在不同的设置中更具用途的应用。Hexagonfab通过引入能够测量原油样品的新一代传感器来扩展其传感器组合。这些传感器具有带有生物分子的电敏感薄膜。一种称为表面功能化的过程。所采用的表面功能化是一种独特的蛋白质/石墨烯复合材料，具有最佳的电导率，同时确保低非特异性结合信号。为了确保成功的产品发射，必须确定实现理想表面功能化的最佳生产和存储条件。这包括测试各种生产方法，然后进行精确的化学分析，以评估表面功能化的特性。该项目与NPL合作，旨在评估不同生产过程对表面功能化的影响。通过识别最有效的过程条件，六边形可以增强其传感器的性能，并确保成功开发高级技术。理解和优化表面涂层过程将确保传感器表现出所需的灵敏度和特异性。凭借其测量原油样品的能力，这些传感器可以直接与粗介质接口，从而可以进行更准确，有效的抗体分析，而无需大量纯化，最终节省了时间和资源。