Pilot HGDP: Efficient Recovery of Useful Markers: Application for use in the Human Genome Diversity Project

试点 HGDP：有效回收有用标记：在人类基因组多样性计划中的应用

• 批准号: 9610505
• 负责人: Glenys Thomson
• 金额: $ 12.41万
• 依托单位: University of California-Berkeley
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-05-01 至 1999-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9610505&HistoricalAwards=false
• 关键词: Pilot; HGDP; Efficient; Recovery; Useful

Abundant genetic variation in humans is widely distributed across the genome. The project of reconstructing human population divergence is best served by those genetic markers differentiating populations. The purpose of this study is to evaluate a large number of microsatellite markers in a sample of human populations for use in a Human Genome Diversity Project (HGDP). Microsatellite loci are regions of repeating DNA sequence. They are utilized for many purposes, including the localization of human disease genes. This study will produce three results pertinent to advancing HGDP goals: a description of the variation (heterozygosity and polymorphism) of several hundred genetic markers among five study populations, the relative informativeness of each marker for studying population divergence, and finally a methodological characterization of each marker for application in diverse laboratory settings. This enormous amount of genetic data will be generated with new methods involving automated typing technology and a novel strategy using pooled DNA samples.

人类丰富的遗传变异广泛分布在整个基因组中。 重建人类群体差异的项目最好由那些区分群体的遗传标记服务。 本研究的目的是评估人类基因组多样性计划（HGDP）中使用的人群样本中的大量微卫星标记。 微卫星位点是重复DNA序列的区域。 它们被用于许多目的，包括人类疾病基因的定位。 这项研究将产生三个结果有关推进HGDP的目标：五个研究人群中的几百个遗传标记的变异（杂合性和多态性）的描述，每个标记的相对信息量研究人口的分歧，最后每个标记在不同的实验室环境中应用的方法学特征。这一巨大数量的遗传数据将通过涉及自动分型技术的新方法和使用合并DNA样本的新策略生成。