Biocatalytic Conversion of Aromatic Waste to Useful Compounds

有了这个环保化学合成和加工项目的更新奖，合成有机项目支持了佛罗里达大学化学系的Tomas Hudlicky博士的研究。Hudlicky教授的工作重点是开发从卤代芳香废物中制备纯合成中间体（合子）的有效方法，并将合子用于简单合成增值化合物。本研究的重点将是利用重组菌株中表达的芳烃双加氧酶对高取代的芳烃底物进行生物氧化，并开发芳烃顺式二醇代谢物进一步氧化为相应的二醇环氧化物、四醇和二醇-氮杂啶的电化学方法。这些新的代谢物和合成子将应用于菊科和吗啡生物碱、糖缀合物和肌醇衍生物的非天然异构体的合成方法。

With this renewal award in the Environmentally Benign Chemical Synthesis and Processing Program, the Synthetic Organic Program is supporting the research of Dr. Tomas Hudlicky of the Department of Chemistry at the University of Florida. Professor Hudlicky will focus his work on developing effective methods of preparation of pure synthetic intermediates (synthons) from halogenated aromatic waste products and the use of the synthons in the concise synthesis of value-added compounds. The focus of this research will be the biooxidation of highly substituted aromatic substrates with arene dioxygenases expressed in recombinant bacterial strains and the development of electrochemical methods for the further oxidation of arene-cis-diol metabolites to the corresponding diol-epoxides, tetrols and diol-aziridines. The new metabolites and synthons will find application in synthetic approaches to Amaryllidaceae and morphine alkaloids, glycocongugates and unnatural isomers of inositol derivatives.