RUI: Molecular Evolution of the Glucose Phosphate Isomerase Locus in Argopecten irradians

RUI：海湾扇贝磷酸葡萄糖异构酶基因座的分子进化

• 批准号: 9615567
• 负责人: Maureen Krause
• 金额: $ 9.09万
• 依托单位: Long Island University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-06-01 至 2000-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9615567&HistoricalAwards=false
• 关键词: RUI; Molecular; Evolution; Glucose; Phosphate

9615567 KRAUSE The degree to which genetic variation for metabolic enzymes can potentially translate into biochemical, physiological and fitness differences among individuals is largely unknown. Previous studies suggest that protein variation for an enzyme involved in energy metabolism from the bay scallop, Argopecten irradians, directly affects biochemical and production-related traits. Scallop size, reproductive output, and certain biochemical traits are associated with genetic variation for the enzyme glucose phosphate isomerase (Gpi). The proposed research will build on previous work and examine DNA sequence variation for the Gpi gene. DNA from individuals with different Gpi protein variants will be sequenced following isolation and amplification by the polymerase chain reaction. A variety of statistical tests will be applied to the nucleotide sequence data in order to test hypotheses regarding the relative strength of random and selective forces maintaining genetic variation at Gpi. Also, the relationships among nucleotide variation and previously characterized protein and size differences among scallops will be explored. The results from this study will contribute to the general body of knowledge describing the relative roles of random and selective forces in evolution. More importantly, these data will begin to address the functional relevance of nucleotide variation and the hypothesis that genetic differences for this glycolytic enzyme translate into fitness differences among individuals in a population.

9615567 KRAUSE 代谢酶的遗传变异在多大程度上可能转​​化为个体之间的生化、生理和健康差异，目前尚不清楚。 先前的研究表明，海湾扇贝（Argopecten irradians）参与能量代谢的酶的蛋白质变异直接影响生化和生产相关性状。 扇贝大小、繁殖产量和某些生化特征与葡萄糖磷酸异构酶 (Gpi) 的遗传变异有关。 拟议的研究将建立在之前的工作基础上，并检查 Gpi 基因的 DNA 序列变异。 来自具有不同 Gpi 蛋白变体的个体的 DNA 将在通过聚合酶链式反应分离和扩增后进行测序。 将对核苷酸序列数据应用各种统计测试，以测试有关维持 Gpi 遗传变异的随机力和选择性力的相对强度的假设。 此外，还将探讨扇贝中核苷酸变异与先前表征的蛋白质和大小差异之间的关系。 这项研究的结果将有助于描述进化中随机力和选择性力的相对作用的一般知识体系。 更重要的是，这些数据将开始解决核苷酸变异的功能相关性以及这种糖酵解酶的遗传差异转化为群体中个体之间的适应性差异的假设。