Tailor-made glycosphingolipids as a toolkit to visualize domain formation and lipid-protein interactions (A05)

定制的鞘糖脂作为可视化结构域形成和脂质-蛋白质相互作用的工具包 (A05)

• 批准号: 107479224
• 金额: --
• 依托单位: Institut für Organische Chemie
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2019-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/107479224?language=en
• 关键词: Tailor; made; glycosphingolipids; toolkit; visualize

The aim of this proposal is to investigate how the ceramide structure of the glycosphingolipid Gb3, the specific receptor for Shiga toxin (STx), influences the spatial distribution and diffusion of the glycolipids and other lipids in lipid bilayers mimicking the outer leaflet of the plasma membrane. We will synthesize head group labeled Gb3 molecules that do not interfere with the binding capability of STx. We will make use of pore spanning membranes and adhered GUVs to investigate STx-induced membrane reorganization also as a function of lateral membrane tension.

本研究的目的是研究志贺毒素（STx）特异性受体鞘糖脂Gb3的神经酰胺结构如何影响糖脂和其他脂类在模拟质膜外小叶的脂质双层中的空间分布和扩散。我们将合成不干扰STx结合能力的头基标记的Gb3分子。我们将利用跨孔膜和粘附的guv来研究stx诱导的膜重组以及膜侧张力的函数。