Tailor-made glycosphingolipids as a toolkit to visualize domain formation and lipid-protein interactions (A05)

本研究的目的是研究志贺毒素(STX)的特异性受体--鞘糖脂Gb3的神经酰胺结构如何影响糖脂和其他脂类在模拟质膜外层小叶的脂双层中的空间分布和扩散。我们将合成不干扰STX结合能力的头部基团标记的Gb3分子。我们将利用跨孔膜和附着的GUV来研究STX诱导的膜重组也是侧膜张力的函数。

The aim of this proposal is to investigate how the ceramide structure of the glycosphingolipid Gb3, the specific receptor for Shiga toxin (STx), influences the spatial distribution and diffusion of the glycolipids and other lipids in lipid bilayers mimicking the outer leaflet of the plasma membrane. We will synthesize head group labeled Gb3 molecules that do not interfere with the binding capability of STx. We will make use of pore spanning membranes and adhered GUVs to investigate STx-induced membrane reorganization also as a function of lateral membrane tension.