Interactions Between Bacterioplankton Communities and Dissolved Substrates at the Bermuda Atlantic Time-Series Study Station

与系统发育组特异性探针的浮游细菌核糖体RNA杂交测量表明，几个主要的浮游细菌组形成高度分层的人口在较低的海洋表层在马尾藻海西北部。 在同一地点的其他研究发现了季节循环的证据，其中溶解有机碳（DOC）在春季积累在上表面层，并在冬季混合输出到下表面层。 通过联合收割机水和不同深度接种物的海水培养试验，研究了浮游细菌群落组成与水体DOC来源、数量和不稳定性的关系。 扩增核糖体RNA基因的高分辨率电泳图将用于调查社区组成。 将通过总群落RNA与系统发育组特异性探针的杂交确认电泳图结果。 这项研究的数据将比较正在进行的测量浮游细菌生产，生物量和DOC的百慕大大西洋时间序列研究的一部分。

Hybridization measurements made with phylogenetic group-specific probes for bacterioplankton ribosomal RNAs have shown that several major bacterioplankton groups form highly stratified populations in the lower ocean surface layer in the northwestern Sargasso Sea. Other studies at the same site have uncovered evidence of a seasonal cycle in which dissolved organic carbon (DOC) accumulates in the upper surface layer during the vernal period and is exported to the lower surface layer during winter mixing. This study proposes to examine the relationship between the composition of bacterioplankton communities and the source, amount and lability of DOC in the water column by conducting seawater culture experiments that combine water and inocula from different depths. High-resolution electropherograms of amplified ribosomal RNA genes will be used to survey community composition. Electropherogram results will be confirmed by hybridizations of total community RNA to phylogenetic group-specific probes. Data from this study will be compared to ongoing measurements of bacterioplankton production, biomass and DOC that are a part of the Bermuda Atlantic Time-Series Study.