The effect of mesoscale eddy perturbations on microbial processes and community structure in the Sargasso Sea

中尺度涡旋扰动对马尾藻海微生物过程和群落结构的影响

• 批准号: 0425615
• 负责人: Craig Carlson
• 金额: $ 34.2万
• 依托单位: University of California-Santa Barbara
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-07-01 至 2008-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0425615&HistoricalAwards=false
• 关键词: effect; mesoscale; eddy; perturbations; microbial

This proposal award will serve as a companion project to the "EDDIES" (EDDY Dynamics, mixing Export, and Species composition) project funded by NSF Biological, Chemical and Physical Oceanography. This interdisciplinary field program will be initiated in the Sargasso Sea during the summer of 2004 and 2005. Recent evidence suggests that mesoscale eddies are an important nutrient transport mechanism in the oligotrophic waters of the main subtropical gyres. Numerical simulations and satellite-based statistical estimates indicate that the magnitude of the eddy-driven nutrient flux could be sufficient to balance geochemical estimates of new production. Relatively few direct observations of this process are available, owing to the spatial and temporal intermittency of the events that drive it. Available data demonstrate that isopycnal displacements associated with certain types of eddies can transport nutrients into the euphotic zone, resulting in the accumulation of chlorophyll in overlying waters. Preliminary data, included in this proposal, indicate that coincident with the passage of a cyclonic eddy at the BATS site, bacterial production rates were significantly enhanced relative to the monthly mean values. However, the extent of the prokaryotic heterotrophic response and their impact on coupled biogeochemical cycles and export has yet to be elucidated. This companion project proposes to sample parameters relevant to heterotrophic prokaryotes across mesoscale eddies documenting how these parameters vary inside the eddy through time and space (horizontal and vertical) relative to outside (.control.) waters. Specifically the PIs propose to: 1. Assess the spatial and temporal variability of prokaryotic biomass and production rates in response to eddy induced nutrient pumping by comparing stocks and rates throughout the surface 700 m, in and out of the mesoscale feature. 2. Determine if a mesoscale eddy perturbation and subsequent nutrient pumping affects the bioavailability of newly produced dissolved organic matter (DOM) and the efficiency with which the prokaryotic community processes it. 3. Assess how eddy induced nutrient pumping may affect the partitioning of attached versus free-living microbial processes. 4. Determine if the mesoscale perturbation and the resulting changes in biogeochemistry lead to a change in the prokaryotic community structure. Intellectual Merit Compilation of these measurements will provide a rigorous assessment of the response of heterotrophic prokaryotes to the organic matter regime of the eddy bloom environment. Prokaryotes play an important role in determining the biogeochemical fate of a bloom. The oligotrophic waters of subtropical gyres are typically characterized by extensive recycling of elements through the microbial loop with little export. As biology responds to the eddy-induced nutrient pumping it will be important to document the rate and efficiency by which heterotrophic prokaryotes process freshly produced organic matter in order to better understand the flow of carbon and energy in these mesoscale features. Monitoring the prokaryotic community structure will be important to gain insight to the ecological response and biogeochemical processing within these features. Our measurements will complement and strengthen concurrent assessments of various parameters collected by the funded EDDIES program. Broader Impacts There are four specific objectives incorporated into the broader impacts portion of this proposal: 1) Participation and support for a post-doctoral fellow as well as undergraduate laboratory assistants, 2) Curricular development at UCSB, 3) Web-based outreach programs integrating the microbial data set into a centralized .EDDIES. website, the BATS and Microbial Observatories websites as well as the UCSB Marine Science Institute web- and laboratory-based outreach programs, and 4) Integration of fieldwork at sea experiences into the UCSB Women in Science and Engineering (WISE) mentorship program targeted at keeping undergraduate women in the sciences.

该提案奖将作为由NSF生物，化学和物理海洋学资助的“EDDYS”（EDDY动力学，混合输出和物种组成）项目的配套项目。 这一跨学科的实地项目将于2004年和2005年夏季在马尾藻海启动。 最近的证据表明，中尺度涡旋是一个重要的营养输送机制，在贫营养沃茨的主要副热带环流。 数值模拟和基于卫星的统计估计表明，涡流驱动的营养通量的大小可能足以平衡新生产的地球化学估计。 由于驱动这一过程的事件在空间和时间上的不连续性，对这一过程的直接观测相对较少，现有数据表明，与某些类型的涡旋有关的等密度线位移可以将营养物质输送到真光层，导致上覆沃茨中叶绿素的积累。初步数据，包括在本提案中，表明，符合在BATS网站的气旋涡流的通道，细菌生产率显着增强相对于月平均值。然而，原核异养反应的程度及其对耦合地球化学循环和出口的影响还有待阐明。这个同伴项目提出了样本参数相关的异养原核生物在中尺度涡记录这些参数如何通过时间和空间（水平和垂直）相对于外部（. control）的涡内变化。沃茨。 具体而言，PI建议：1. 通过比较整个表面700米的存量和速率，评估原核生物量和生产率的空间和时间变异性，以响应涡流引起的营养物泵送，进出中尺度特征。 2. 确定是否中尺度涡扰动和随后的营养泵影响新产生的溶解有机物（DOM）的生物利用度和原核生物群落处理它的效率。 3. 评估涡流诱导的营养泵如何影响附着与自由生活的微生物过程的分区。4. 确定中尺度扰动和由此产生的地球化学变化是否导致原核生物群落结构的变化。 智力优势 这些测量的汇编将提供一个严格的评估响应的异养原核生物的涡流水华环境的有机物制度。原核生物在决定水华的生态地球化学命运方面起着重要作用。亚热带环流贫营养沃茨的典型特征是通过微生物循环进行大量元素再循环，但几乎没有输出。由于生物学对涡流引起的营养泵的响应，因此记录异养原核生物处理新鲜产生的有机物的速率和效率以更好地理解这些中尺度特征中的碳和能量流动将是重要的。 监测原核生物群落结构将是重要的，以了解这些功能内的生态响应和生物地球化学处理。我们的测量将补充和加强对受资助的EDCAND计划收集的各种参数的同步评估。 更广泛的影响有四个具体目标纳入本提案的更广泛的影响部分：1）参与和支持博士后研究员以及本科生实验室助理，2）UCSB的课程开发，3）基于Web的外展计划将微生物数据集整合到一个集中的. EDDIES。网站、BATS和微生物观测站网站以及UCSB海洋科学研究所基于网络和实验室的外展计划; 4）将海上实地工作经验纳入UCSB科学与工程女性（WISE）导师计划，旨在让本科女性留在科学领域。