Primary lipid vesicle fusion events: Pathways, forces, energetics (B02)

原发性脂质囊泡融合事件：途径、力、能量学 (B02)

• 批准号: 108215615
• 金额: --
• 依托单位: Max-Planck-Institut für biophysikalische Chemie (Karl-Friedrich-Bonhoeffer-Institut) (aufgelöst)
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2019-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/108215615?language=en
• 关键词: Primary; lipid; vesicle; fusion; events

Fusion of lipid vesicles and membranes is a fundamental intra- and intercellular transport mechanism, mediated, controlled, and triggered by a complex machinery of fusion proteins. By atomistic simulations, we will calculate the free energy profiles along the fusion pathways determined during the past funding period, with a particular focus on the hemifusion and subsequent pore expansion steps. To structurally interpret the experimentally determined fusion kinetics, we will further use a generalized Helfrich model to disentangle which parts of the pore dictate the energetics and kinetics of the involved topological changes.

脂质囊泡和膜的融合是一种基本的细胞内和细胞间转运机制，由融合蛋白的复杂机制介导、控制和触发。通过原子模拟，我们将计算自由能分布沿着在过去的资助期间确定的融合途径，特别侧重于半融合和随后的孔扩张步骤。为了从结构上解释实验确定的融合动力学，我们将进一步使用广义Helfrich模型来解开哪些部分的孔决定所涉及的拓扑变化的能量学和动力学。