ENGINEERING RESEARCH EQUIPMENT: A Combined Fluorescence Optical Microscope/Non-Contact Atomic Force Microscope for Monolayer and Multilayer Studies
工程研究设备:用于单层和多层研究的组合荧光光学显微镜/非接触原子力显微镜
基本信息
- 批准号:9622506
- 负责人:
- 金额:$ 5万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Standard Grant
- 财政年份:1996
- 资助国家:美国
- 起止时间:1996-05-15 至 1998-04-30
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
9622506 Zasadzinski New modes of imaging with scanning probe microscopes - non-contact, tapping, and friction or lateral force modes - have made it possible to image a variety of surfaces that had previously been difficult or impossible to OseeO. These new imaging modes take advantage of variations in surface chemistry, friction, compliance, charge, etc. to develop image contrast in addition to simple surface topography. For many of these modes, the AFM tip is either not in contact with or is gently tapped on the sample surface. Hence, much softer surfaces can be imaged than with conventional contact mode AFM and there is less tendency to rearrange loosely supported materials. It is proposed to upgrade the current contact mode AFM by purchasing a Nanoscope Multimode AFM and the necessary accessories to do non-contact and tapping mode imaging. These new imaging modalities will be applied to the ongoing studies of self-assembly and pattern formation in surfactant, lipid and proteins. These new imaging modes are proving to be extremely useful in examining complex fluid interfaces, surfactant, protein, and polymer adsorption, lateral phase separation in membranes, protein localization and diffusion, the kinetics and structures of self-assembled films, etc. This new apparatus will also allow us to characterize more realistic model membrane surfaces for force measurements with the Surface Forces Apparatus. To fully implement the new imaging modes, it is necessary to identify areas of interest on the surface prior to engaging the AFM. The best way to do this is by coupling the AFM to a high resolution optical microscope capable of fluorescence imaging. Fluorescence microscopy is one of the best ways of identifying lipid domains, specific proteins (via antibody labeling), adsorption etc. at the micron level. It would be a major advance to directly correlate the fluorescence images with molecular resolution AFM images. A custom fluorescence microscope has recently been built for examin ing monolayers at the air-water interface. A very similar instrument can be constructed to examine thin films on substrates prior to and during AFM imaging. With this combined capability, the principal investigator should be able to localize proteins, phase boundaries, etc. and correlate them with their macroscopic effects on bilayer properties. The transfer of the spontaneous patterns formed by various lipid mixtures at the air-water interface to substrates to be used as lithographic-style templates, the patterns formed by competitive self-assembly of mixtures of adsorbing species, and the initial stages of mineralization of surfaces, can also be studied. ***
小行星9622506 扫描探针显微镜成像的新模式-非接触、轻敲和摩擦或横向力模式-使得可以对以前难以或不可能的各种表面进行成像。 这些新的成像模式利用表面化学、摩擦、顺应性、电荷等的变化,除了简单的表面形貌之外,还形成图像对比度。 对于这些模式中的许多模式,AFM针尖要么不与样品表面接触,要么轻轻地敲击样品表面。 因此,软得多的表面可以成像比传统的接触模式AFM和有较少的倾向,重新安排松散的支持材料。 建议通过购买Nanoscope Multimode AFM和必要的配件来升级当前的接触式AFM,以进行非接触和轻敲模式成像。 这些新的成像模式将被应用到正在进行的表面活性剂,脂质和蛋白质的自组装和模式形成的研究。 这些新的成像模式被证明是非常有用的,在检查复杂的流体界面,表面活性剂,蛋白质和聚合物的吸附,横向相分离膜,蛋白质的定位和扩散,动力学和自组装膜的结构等,这种新的设备也将使我们能够表征更现实的模型膜表面的表面力装置的力测量。 为了完全实现新的成像模式,有必要在使用AFM之前识别表面上的感兴趣区域。 最好的方法是将AFM与能够进行荧光成像的高分辨率光学显微镜耦合。 荧光显微镜是在微米级识别脂质结构域、特定蛋白质(通过抗体标记)、吸附等的最佳方法之一。 这将是一个重大的进步,直接相关的荧光图像与分子分辨率的AFM图像。 一个定制的荧光显微镜最近已经建成检查单分子层在空气-水界面。 一个非常相似的仪器可以被构造成在AFM成像之前和期间检查衬底上的薄膜。 有了这种综合能力,主要研究者应该能够定位蛋白质,相边界等,并将它们与它们对双层性质的宏观影响相关联。 各种脂质混合物在空气-水界面上形成的自发图案转移到基板上用作平版印刷模板,通过吸附物种的混合物的竞争性自组装形成的图案,以及表面矿化的初始阶段,也可以进行研究。 ***
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Joseph Zasadzinski其他文献
Effect of Hydrophobic Surfactant Proteins SP-B and SP-C on the Phase and Morphology of Protein Deficient Native Surfactant Films
- DOI:
10.1016/j.bpj.2009.12.503 - 发表时间:
2010-01-01 - 期刊:
- 影响因子:
- 作者:
Prajnaparamita Dhar;Joseph Zasadzinski - 通讯作者:
Joseph Zasadzinski
Studying the In Vivo Behavior of the Vesosome
- DOI:
10.1016/j.bpj.2008.12.2320 - 发表时间:
2009-02-01 - 期刊:
- 影响因子:
- 作者:
Benjamin Wong;Jason Schmidt;Joseph Zasadzinski - 通讯作者:
Joseph Zasadzinski
Alterations In Phase And Morphology Of A Lung Surfactant Monolayer in contact with surfactant in the sub-phase induced by cholesterol and native surface active proteins
- DOI:
10.1016/j.bpj.2008.12.3229 - 发表时间:
2009-02-01 - 期刊:
- 影响因子:
- 作者:
Prajnaparamita Dhar;Patrick Stenger;Joseph Zasadzinski - 通讯作者:
Joseph Zasadzinski
The Progression of a Novel Liposome-Based Delivery Vehicle Toward in vivo Drug Delivery
- DOI:
10.1016/j.bpj.2009.12.3673 - 发表时间:
2010-01-01 - 期刊:
- 影响因子:
- 作者:
Benjamin Wong;Jason Schmidt;Joseph Zasadzinski - 通讯作者:
Joseph Zasadzinski
Joseph Zasadzinski的其他文献
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{{ truncateString('Joseph Zasadzinski', 18)}}的其他基金
Effects of Curvature on Monolayer Morphology and Dynamics
曲率对单层形态和动力学的影响
- 批准号:
1706378 - 财政年份:2017
- 资助金额:
$ 5万 - 项目类别:
Standard Grant
Collaborative Research in Nanostructure Control via Surfactant Mixing and Polymerization
通过表面活性剂混合和聚合控制纳米结构的合作研究
- 批准号:
0436124 - 财政年份:2005
- 资助金额:
$ 5万 - 项目类别:
Standard Grant
Acquisition of a High Vacuum Freeze-Fracture System for Microstructural Characterization of Complex Fluids and Biomaterials
获得用于复杂流体和生物材料微观结构表征的高真空冷冻断裂系统
- 批准号:
9802591 - 财政年份:1998
- 资助金额:
$ 5万 - 项目类别:
Standard Grant
Engineering Research Equipment: A Modified STM/AFM for Complex Fluid Investigations
工程研究设备:用于复杂流体研究的改进型 STM/AFM
- 批准号:
9212790 - 财政年份:1992
- 资助金额:
$ 5万 - 项目类别:
Standard Grant
Presidential Young Investigator Award: Surfactant Solution Properties and Fluid Microstructure
总统青年研究员奖:表面活性剂溶液特性和流体微观结构
- 批准号:
8657444 - 财政年份:1987
- 资助金额:
$ 5万 - 项目类别:
Continuing Grant
Acquisition of an Electron Microscope (Materials Research)
购买电子显微镜(材料研究)
- 批准号:
8719771 - 财政年份:1987
- 资助金额:
$ 5万 - 项目类别:
Standard Grant
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