VPW: Thio Derivatives of Nuclei Acids for the Study of Metal Ion Binding Sites and RNA Structure

VPW：用于金属离子结合位点和 RNA 结构研究的核酸硫代衍生物

• 批准号: 9627113
• 负责人: Janet Morrow
• 金额: $ 11.02万
• 依托单位: University of Rochester
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-08-15 至 1998-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9627113&HistoricalAwards=false
• 关键词: VPW; Thio; Derivatives; Nuclei; Acids

The long term goal of this research is to use thio derivatives of RNA bases and phosphate diesters to explore or to exploit metal ion binding on nucleic acids. The first project uses a single phosphorothioate modification to direct a metal ion cross-linking agent to a Tetrahymena ribozyme for the purpose of studying ribozyme structure and selective inactivation of RNA. The second project examines metal ion binding to 4-thiouridine and 6- thioguanosine and incorporate a 4-thiouridine into a Hammerhead ribozyme in positions thought to be important in metal ion binding. Metal-ion-RNA interactions are a key component of RNA structure and are important in the development of therapeutic agents for the inactivation of RNA. Interactive activities in the classroom and in the research laboratory involve both undergraduate and graduate female chemistry students. A graduate level interdisciplinary course in bioinorganic chemistry is being taught, and a seminar series is being run on careers in the physical sciences in order to induce female students to consider physical science degrees.

这项研究的长期目标是使用RNA碱基和磷酸二酯的硫代衍生物来探索或利用金属离子与核酸的结合。第一个项目使用单一的硫代修饰将金属离子交联剂定向到四膜虫核酶上，目的是研究核酶结构和RNA的选择性失活。第二个项目研究金属离子与4-硫代尿苷和6-硫代鸟苷的结合，并在被认为对金属离子结合很重要的位置将4-硫代尿苷结合到锤头状核酶中。金属-离子-RNA相互作用是RNA结构的重要组成部分，在RNA灭活治疗药物的开发中具有重要意义。课堂和研究实验室的互动活动涉及化学本科生和研究生。正在教授生物无机化学的研究生级跨学科课程，并正在举办一系列关于物理科学职业的研讨会，以鼓励女学生考虑物理科学学位。