RPG: Origin of Retrotransposon RNA Polymerase III Transcripts in Drosophila

RPG：果蝇逆转录转座子 RNA 聚合酶 III 转录本的起源

• 批准号: 9629741
• 负责人: Irina Arkhipova
• 金额: $ 1.8万
• 依托单位: Harvard University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-07-15 至 1997-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9629741&HistoricalAwards=false
• 关键词: RPG; Origin; Retrotransposon; RNA; Polymerase

Arkhipova 96-29741 Research Planning Grant Abstract This project focuses on a Drosophila melanogaster moderately repetitive retrotransposon, mdg1, with an exceptionally complex promoter, the activity of which can be changed during development of the fly. A novel aspect which has never been observed in vitro is its duel specificity for RNA polymerase II/III (pol II/III). The generality and the basis of this phenomenon need be investigated but preliminary studies are needed to define the two possible origins of pol II/III transcripts of the retrotransposon and to detect any variation in transcripts during the development of the fly. These results will allow further research in one of two alternate directions. %%% Participation of mobile DNA in the organization and function of eukaryotic genomes remains a subject of controversy. However it is beginning to be recognized that many different roles might be played in various processes at the cellular, organismic and population levels by different transposons. Transposons share the corresponding machinery with many host genes and their ability to multiply can lead to multiplication of regulatory elements found in the transposons. Therefore, a knowledge of the diverse regulatory sequences carried by transposons, such as promoters, could result in understanding of transposon involvement into qualitative as well as quantitative aspects of regulatory evolution in higher eukaryotic organisms.

Arkhipova 96-29741研究计划赠款摘要该项目着重于果蝇Melanogaster中等重复的Retrotransposon，MDG1，其中具有异常复杂的启动子，其活动在飞行过程中可以改变。从未在体外观察到的新方面是其对RNA聚合酶II/III（POL II/III）的决斗特异性。需要研究这种现象的一般性和基础，但是需要初步研究来定义逆转录跨座子的POL II/III转录本的两个可能起源，并检测苍蝇发育过程中转录本的任何变化。这些结果将允许在两个替代方向之一中进行进一步研究。 移动DNA参与真核基因组的组织和功能仍然是一个争议的主题。但是，人们开始认识到，不同的转座子在细胞，有机体和人群水平的各种过程中可能会发挥许多不同的作用。转座子与许多宿主基因共享相应的机械，它们的繁殖能力可以导致转座中发现的调节元件的繁殖。因此，对转座子（例如启动子）携带的多种调节序列的了解可能会导致理解转座子的参与到较高真核生物体中调节性进化的定性和定量方面。