RPG: Origin of Retrotransposon RNA Polymerase III Transcripts in Drosophila

Arkhipova 96-29741研究计划资助摘要这个项目关注的是果蝇的一种适度重复的反转录转座子mdg1，它有一个异常复杂的启动子，其活性可以在果蝇的发育过程中改变。一个从未在体外观察到的新方面是它对RNA聚合酶II/III(PolII/III)的双重专一性。需要研究这种现象的普遍性和基础，但需要进行初步研究，以确定反转录转座子PolII/III转录本的两个可能来源，并检测果蝇发育过程中转录本的任何变异。这些结果将允许在两个不同方向中的一个方向进行进一步研究。移动DNA在真核基因组的组织和功能中的参与仍然是一个有争议的主题。然而，人们开始认识到，不同的转座子可能在细胞、组织和种群水平的不同过程中发挥许多不同的作用。转座子与许多寄主基因共享相应的机制，它们的增殖能力可以导致转座子中发现的调控元件的倍增。因此，了解转座子携带的不同调控序列，如启动子，可能会导致理解转座子参与高等真核生物调控进化的定性和定量方面。

Arkhipova 96-29741 Research Planning Grant Abstract This project focuses on a Drosophila melanogaster moderately repetitive retrotransposon, mdg1, with an exceptionally complex promoter, the activity of which can be changed during development of the fly. A novel aspect which has never been observed in vitro is its duel specificity for RNA polymerase II/III (pol II/III). The generality and the basis of this phenomenon need be investigated but preliminary studies are needed to define the two possible origins of pol II/III transcripts of the retrotransposon and to detect any variation in transcripts during the development of the fly. These results will allow further research in one of two alternate directions. %%% Participation of mobile DNA in the organization and function of eukaryotic genomes remains a subject of controversy. However it is beginning to be recognized that many different roles might be played in various processes at the cellular, organismic and population levels by different transposons. Transposons share the corresponding machinery with many host genes and their ability to multiply can lead to multiplication of regulatory elements found in the transposons. Therefore, a knowledge of the diverse regulatory sequences carried by transposons, such as promoters, could result in understanding of transposon involvement into qualitative as well as quantitative aspects of regulatory evolution in higher eukaryotic organisms.