Plant Genes Involved in Agrobacterium-Mediated Transformation

参与农杆菌介导转化的植物基因

• 批准号: 9630779
• 负责人: Stanton Gelvin
• 金额: $ 36万
• 依托单位: Purdue Research Foundation
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-08-01 至 1999-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9630779&HistoricalAwards=false
• 关键词: Plant; Genes; Involved; Agrobacterium; Mediated

9630779 Gelvin Agrobacterium tumefaciens causes the disease Crown Gall on many species of plants. The molecular mechanism of Crown Gall tumorigenesis involves the binding of bacterial cells to the plant cell wall, and the processing of a region of the Ti-(tumor inducing) plasmid and transfer of this T-(transfer) DNA to plant cells. Ti plasmid has been modified to be used as a vector for genetic engineering in plants. As a result of two decades of research, we know much about the contribution of bacterial genes and proteins to T-DNA transfer and tumorigenesis. However, we know little about the role plant genes and proteins play in these processes. In an attempt to identify host-encoded factors necessary for Agrobacterium binding, T-DNA transfer and integration, and phytohormone signal transduction, Dr. Gelvin has recently screened different Arabidopsis thaliana ecotypes and T-DNA insertion mutants for susceptibility to crown gall tumorigenesis, as well as the ability to express other T-DNA-encoded functions both transiently and stably. In this research project, Dr. Gelvin's proposes experiments to identify specifically the steps of crown gall tumorigenesis that are blocked in Arabidopsis T-DNA insertion mutants that have already been identified. The characterization of these host genes will not only increase our understanding of crown gall tumorigenesis, but will also contribute to our knowledge of basic plant cell biology. Finally, an understanding and the isolation of the host genes involved in these processes may allow us to manipulate these species so that they become more amenable to T-DNA-mediated genetic transformation.

9630779根癌农杆菌引起多种植物的冠瘿病。冠瘿瘤发生的分子机制涉及细菌细胞与植物细胞壁的结合，以及Ti-（肿瘤诱导）质粒的一个区域的加工和T-（转移）DNA向植物细胞的转移。钛质粒已被修饰为植物基因工程的载体。经过二十年的研究，我们对细菌基因和蛋白质在T-DNA转移和肿瘤发生中的作用有了更多的了解。然而，我们对植物基因和蛋白质在这些过程中所起的作用知之甚少。为了鉴定农杆菌结合、T-DNA转移和整合以及植物激素信号转导所必需的宿主编码因子，Gelvin博士最近筛选了不同的拟南芥生态型和T-DNA插入突变体对冠瘿瘤发生的易感性，以及瞬时和稳定表达其他T-DNA编码功能的能力。在这个研究项目中，Gelvin博士提出了实验，以确定已经确定的拟南芥T-DNA插入突变体中被阻断的冠瘿瘤发生的具体步骤。这些寄主基因的鉴定不仅将增加我们对冠瘿瘤发生的认识，而且将有助于我们了解基础植物细胞生物学。最后，了解和分离参与这些过程的宿主基因可能使我们能够操纵这些物种，使它们更容易接受t - dna介导的遗传转化。