Plant Genes Involved in Agrobacterium-Mediated Transformation

参与农杆菌介导转化的植物基因

• 批准号: 9630779
• 负责人: Stanton Gelvin
• 金额: $ 36万
• 依托单位: Purdue Research Foundation
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-08-01 至 1999-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9630779&HistoricalAwards=false
• 关键词: Plant; Genes; Involved; Agrobacterium; Mediated

9630779 Gelvin Agrobacterium tumefaciens causes the disease Crown Gall on many species of plants. The molecular mechanism of Crown Gall tumorigenesis involves the binding of bacterial cells to the plant cell wall, and the processing of a region of the Ti-(tumor inducing) plasmid and transfer of this T-(transfer) DNA to plant cells. Ti plasmid has been modified to be used as a vector for genetic engineering in plants. As a result of two decades of research, we know much about the contribution of bacterial genes and proteins to T-DNA transfer and tumorigenesis. However, we know little about the role plant genes and proteins play in these processes. In an attempt to identify host-encoded factors necessary for Agrobacterium binding, T-DNA transfer and integration, and phytohormone signal transduction, Dr. Gelvin has recently screened different Arabidopsis thaliana ecotypes and T-DNA insertion mutants for susceptibility to crown gall tumorigenesis, as well as the ability to express other T-DNA-encoded functions both transiently and stably. In this research project, Dr. Gelvin's proposes experiments to identify specifically the steps of crown gall tumorigenesis that are blocked in Arabidopsis T-DNA insertion mutants that have already been identified. The characterization of these host genes will not only increase our understanding of crown gall tumorigenesis, but will also contribute to our knowledge of basic plant cell biology. Finally, an understanding and the isolation of the host genes involved in these processes may allow us to manipulate these species so that they become more amenable to T-DNA-mediated genetic transformation.

9630779根癌农杆菌会在许多植物上引起冠瘤。冠瘤发生的分子机制包括细菌细胞与植物细胞壁的结合，以及钛(肿瘤诱导)质粒区域的加工和T-转移DNA到植物细胞的转移。经修饰后的TI质粒可作为植物基因工程的载体。经过二十年的研究，我们对细菌基因和蛋白质在T-DNA转移和肿瘤发生中的作用有了很多了解。然而，我们对植物基因和蛋白质在这些过程中扮演的角色知之甚少。为了确定农杆菌结合、T-DNA转移和整合以及植物激素信号转导所必需的寄主编码因子，Gelvin博士最近筛选了不同的拟南芥生态型和T-DNA插入突变体，以研究其对冠瘤发生的易感性，以及瞬时和稳定表达其他T-DNA编码功能的能力。在这项研究项目中，盖尔文博士提议进行实验，以明确识别在已被识别的拟南芥T-DNA插入突变体中受阻的冠瘤发生的步骤。这些寄主基因的特征不仅将增加我们对冠癌发生的理解，还将有助于我们对植物细胞生物学的基础知识的了解。最后，对这些过程中涉及的宿主基因的了解和分离可能会使我们能够操纵这些物种，使它们变得更容易接受T-DNA介导的遗传转化。