Na and Ca Transport Across the Plasma Membrane of Squid Giant Axons: Mechanism of Metabolic Control of the Na/Ca Exchanger

钠和钙跨鱿鱼巨轴突质膜的运输：钠/钙交换器的代谢控制机制

• 批准号: 9631107
• 负责人: Luis Beauge
• 金额: $ 12.45万
• 依托单位: Marine Biological Laboratory
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-08-01 至 2000-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9631107&HistoricalAwards=false
• 关键词: Na; Ca; Transport; Across; Plasma

9631107 Beauge Calcium (Ca) ions act as transducers of the information reaching the cell surface into a language known by the cell interior in nearly all animal cells. Changes in intracellular calcium concentrations is the signal that activates functions like hormone secretion, muscle contraction, and nerve-cell communication. The actual signal is a large increase in the concentration of the intracellular Ca, after which the Ca concentration returns rapidly to the "resting" levels. The research funded by this grant is directed at one of the mechanisms responsible for the "recovery" of resting Ca concentration levels - sodium (Na) ion/calcium ion exchange. The emphasis is on the control of this exchange system by two phosphoryl group donors: ATP and phosphoarginine. This work is carried out on a giant axon in the squid where the internal and external environments can be controlled by the experimenters.. Understanding the mechanisms by which Na-Ca exchange is regulated is crucial for understanding the mechanisms that underlie neural signaling in all animals. In addition, it is crucial for understanding muscle contraction.

在几乎所有的动物细胞中，钙离子作为到达细胞表面的信息的换能器，转换成细胞内部已知的语言。细胞内钙浓度的变化是激活激素分泌、肌肉收缩和神经细胞通讯等功能的信号。实际信号是细胞内钙浓度的大幅增加，之后钙浓度迅速恢复到“静息”水平。该基金资助的研究是针对负责“恢复”静息钙浓度水平的机制之一-钠（Na）离子/钙离子交换。重点是两个磷酸基供体：ATP和磷酸甘氨酸对这个交换系统的控制。这项工作是在鱿鱼的一个巨大的轴突上进行的，在那里实验者可以控制内部和外部环境。了解Na-Ca交换的调节机制对于理解所有动物的神经信号传导机制至关重要。此外，它对于理解肌肉收缩也是至关重要的。