Ca and Na Transport in Heart Failure

心力衰竭中的钙和钠转运

• 批准号: 7078648
• 负责人: Donald M Bers
• 金额: $ 48.72万
• 依托单位: LOYOLA UNIVERSITY CHICAGO
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-07-05 至 2009-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7078648
• 关键词: arrhythmia; calcium channel; calcium flux; calcium ion; calmodulin dependent protein kinase; cardiac myocytes; clinical research; disease /disorder model; genetically modified animals; heart electrical activity; heart failure; heart pharmacology; human tissue; idiopathic dilated cardiomyopathy; ion transport; laboratory mouse; laboratory rabbit; patient oriented research; phospholamban; phosphoproteins; phosphorylation; protein kinase A; sarcoplasmic reticulum; sodium channel; sodium ion; sodium potassium exchanging ATPase

DESCRIPTION (provided by applicant): Heart failure (HF) is a major cause of death in the US. A central aspect is reduced cardiac contractility, and much evidence indicates that altered myocyte Ca handling, particularly reduced SR Ca content is centrally responsible. Myocyte Ca & Na regulation are tightly linked by Na/Ca exchange (which we have studied in depth). Our overall goal is to understand altered Ca & Na regulation in HF. We focus on in-depth analysis of key aspects of SR Ca handling (Aim 1 & 2) and myocyte Na transport (Aim 3 & 4), including basic mechanistic & quantitative issues in normal myocytes, and also how these change in HF. We will use our well characterized nonischemic rabbit HF model (& human myocytes) with key mechanistic studies using transgenic and knockout mice. Using interdigitated fluorescence, confocal, electrophysiological and biochemical approaches, we will address 4 issues: 1. Intra-SR free [Ca] ([Ca]sR). Using our new method to directly measure (Ca)sR,we will test a) if important spatial (Ca)sR gradients exist, b) why SR Ca load is low in HF (low (Ca)sR, SR volume or Ca buffering), c) if phospholamban (PLB) reduces SR Ca-ATPase efficiency, and d) how (Ca)sa may dynamically function in terminating SR Ca release during E-C coupling. 2. SR Ca leak in HF and PKA & CaMKII effects. Diastolic SR Ca leak in HF and protein kinase effects are controversial. We will use Ca sparks & our novel method to measure leak. We will clarify how leak is altered in HF as a function of SR Ca load, and how PKA and CaMKII modulate SR Ca leak in control & HF myocytes (including changes in expression & phosphorylation state of SR proteins in HF. 3. Na influx in HF. (Na)+ is elevated in HF and we showed that elevated TTX-sensitive resting Na influx is largely responsible (e.g. vs. Na/H or Na/Ca exchange). We will test whether this is also true during stimulation in HF, and whether the TTX-sensitive Na influx in HF is attributable to slowly inactivating or window Na current. 4. Phospholemman (PLM) and Na/K-ATPase modulation. PLM is an endogenous regulator of Na/K-ATPase, and a major PKA target in heart. We will test hypotheses that a) endogenous PLM inhibits Na/K-ATPase, and that inhibition is relieved by PKA-dependent phosphorylation, b) PLM expression level modulate Na/K-ATPase expression, and c) in HF, lower PLM expression and/or higher phosphorylation explain why lower Na/K-ATPase expression in HF does not depress Na-pump function. These studies will interweave both quantitative fundamental mechanistic studies of cardiac myocyte E-C coupling, Ca and Na regulation in control and HF (regarding SR Ca transport, how SRCa release shuts off during E-C coupling, PKA & CaMKII effects, Na influx, NCX and Na/K-ATPase). We will test explicit mechanistic hypotheses which will enrich our fundamental understanding of Ca and Na regulation in heart cells, but also provide new insight into how these are altered in HF. This should help in developing new therapeutic targets and strategies for the treatment of human HF.

描述（由申请人提供）：心力衰竭（HF）是美国死亡的主要原因。中心方面是心脏收缩力降低，许多证据表明心肌细胞钙处理的改变，特别是SR钙含量的降低是主要原因。肌细胞Ca和Na的调节与Na/Ca交换密切相关（我们对此进行了深入研究）。我们的总体目标是了解HF中Ca和Na调节的改变。我们专注于深入分析SR Ca处理（Aim 1和Aim 2）和肌细胞Na运输（Aim 3和Aim 4）的关键方面，包括正常肌细胞的基本机制和定量问题，以及这些在HF中的变化。我们将使用我们的非缺血性兔HF模型（和人类肌细胞），并使用转基因和敲除小鼠进行关键的机制研究。利用交叉指间荧光、共聚焦、电生理和生化方法，我们将解决4个问题：