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Control of bacterial biofilm communities by protozoans under semi-natural conditions

半自然条件下原生动物对细菌生物膜群落的控制

基本信息

批准号：
110653204
负责人：
Professor Dr. Markus Weitere
金额：
--
依托单位：
Department Fließgewässerökologie
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2009
资助国家：
德国
起止时间：
2008-12-31 至 2011-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/110653204?language=en
关键词：
Control bacterial biofilm communities protozoans

项目摘要

Biofilms, the complex communities of microbiota in association with solid substrates, play an important role in the matterflux of aquatic ecosystems. In the course of the present study it is planned to analyze for the first time the role of unicellular grazers (protozoans) in controlling the structure of biofilm-dwelling bacteria communities under semi-natural conditions. The biofilms will be established in micro-chambers ( flow cells ) and inoculated by either natural water from the River Rhine (the grazer composition will be manipulated by size fractionation here) or by field isolates of complex bacterial communities in combination with the controlled addition of specific grazers. These different approaches will be applied to test the effects of natural grazer communities with different complexities (containing either flagellates as first order-consumers alone, or additional ciliates as higher-order consumers), or the effects of specific grazer species. Different stages of the biofilm development will be considered. The morphological structure of the bacterial communities will be analyzed by confocal laser-scanning microscopy, its composition by a DNA-fingerprinting method (DGGE) in combination with sequencing of relevant bands. The expected results on the control of bacterial biofilms are relevant for different research areas including environmental and applied microbial ecology.

生物膜是与固体基质相结合的微生物群落，在水生态系统的物质流中起着重要的作用。在本研究的过程中，计划分析的单细胞食草动物（原生动物）在控制半自然条件下的生物膜居住的细菌群落结构的作用。将在微室（流动池）中建立生物膜，并通过来自莱茵河的天然水（此处将通过大小分级操作食草动物组成）或通过复杂细菌群落的田间分离物结合特定食草动物的受控添加进行接种。这些不同的方法将被应用到测试的影响，自然食草动物群落具有不同的复杂性（包含鞭毛虫作为第一阶消费者单独，或额外的纤毛虫作为高阶消费者），或特定的食草动物物种的影响。将考虑生物膜发展的不同阶段。将通过共聚焦激光扫描显微镜分析细菌群落的形态结构，通过DNA指纹法（DGGE）结合相关条带测序分析其组成。控制细菌生物膜的预期结果与不同的研究领域有关，包括环境和应用微生物生态学。