Quorum sensing control of bacterial biofilm formation and dispersal

细菌生物膜形成和扩散的群体感应控制

• 批准号: 1713731
• 负责人: Bonnie Bassler
• 金额: $ 61万
• 依托单位: Princeton University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-08-01 至 2021-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1713731&HistoricalAwards=false
• 关键词: Quorum; sensing; control; bacterial; biofilm

The overall goal of the research is to understand quorum sensing, the cell-to-cell communication process that controls bacterial collective behaviors. The studies proposed here will explore quorum-sensing control of single-species and mixed-species biofilm formation and dispersal. Biofilms are surface-attached bacterial communities and they represent the predominant form of bacterial life on Earth. Bacterial biofilms are crucial for ecology, industry, and medicine. This work will advance the progress of science by revealing the mechanisms that provide these multicellular systems their resilience. These investigations could lead to synthetic strategies for controlling bacterial biofilm formation and dispersal, when biofilm formation negatively impacts the environment and human health. Students at all levels will be trained by working on this project. Efforts to promote diversity among science faculty and students will continue, as will outreach efforts to inform the public across the nation.Biofilms are communities of cells adhered to surfaces. Biofilms represent the predominant form of bacterial life on Earth. Bacterial biofilms are crucial for ecology, industry, and medicine. This project will develop new imaging tools capable of resolving the growth dynamics and gene expression patterns of individual bacterial cells inside living biofilms. Gene regulation programs will be defined to understand what drives the spatial and temporal organization of biofilms and what mechanisms provide these multicellular systems their resilience. Other goals will be to discover how quorum sensing controls development of clonal and non-clonal biofilms, to reveal the molecular mechanisms driving transitions between the biofilm growth mode and the free-swimming state and to explore the ecological consequences to bacteria of transitioning into and out of biofilms. At the most general level, the research should move quorum-sensing studies out of the realm of shaken flask cultures and into new territory that includes realistic spatial and temporal features that more closely resemble the conditions under which bacteria normally live. Consequently, the results should reveal the principles underpinning the evolution and maintenance of expensive traits that are not essential under laboratory conditions, but are crucial and precisely regulated in nature. With this knowledge in hand, the field should be able to rapidly move forward to develop synthetic strategies to prevent or promote quorum sensing, to prevent or promote single-species and mixed-species biofilm formation, and to prevent or promote biofilm dispersal, as required.

这项研究的总体目标是了解群体感应，这是控制细菌集体行为的细胞间通信过程。本文提出的研究将探索单一物种和混合物种生物膜形成和扩散的群体感应控制。生物膜是附着在表面的细菌群落，它们代表了地球上细菌生命的主要形式。细菌生物膜对生态学、工业和医学都是至关重要的。这项工作将通过揭示为这些多细胞系统提供弹性的机制来推动科学进步。当生物膜的形成对环境和人类健康造成负面影响时，这些研究可能导致控制细菌生物膜形成和扩散的综合策略。所有级别的学生都将通过参与这个项目来接受培训。促进科学教职员工和学生多样性的努力将继续下去，向全国公众宣传的宣传努力也将继续。生物膜是附着在表面上的细胞群落。生物膜是地球上细菌生命的主要形式。细菌生物膜对生态学、工业和医学都是至关重要的。该项目将开发新的成像工具，能够解析活生物膜内单个细菌细胞的生长动力学和基因表达模式。基因调控计划将被定义为了解是什么驱动了生物膜的空间和时间组织，以及什么机制为这些多细胞系统提供了弹性。其他目标将是发现群体感应如何控制克隆和非克隆生物膜的发育，揭示推动生物膜生长模式和自由游泳状态转换的分子机制，并探索转换进入和退出生物膜对细菌的生态后果。在最一般的层面上，这项研究应该将群体感应研究从摇瓶培养的领域转移到新的领域，其中包括更接近细菌正常生存条件的现实的空间和时间特征。因此，结果应该揭示支撑昂贵特征进化和维持的原理，这些特征在实验室条件下并不是必不可少的，但在自然界中是关键的和精确的调控。有了这些知识，该领域应该能够迅速向前推进，根据需要制定防止或促进群体感应、防止或促进单一物种和混合物种生物膜形成以及防止或促进生物膜扩散的综合战略。

项目成果

Hierarchical transitions and fractal wrinkling drive bacterial pellicle morphogenesis.

• DOI: 10.1073/pnas.2023504118
• 发表时间: 2021-05-18
• 期刊: Proceedings of the National Academy of Sciences of the United States of America
• 影响因子: 11.1
• 作者: Qin B;Fei C;Wang B;Stone HA;Wingreen NS;Bassler BL
• 通讯作者: Bassler BL

Quorum sensing across bacterial and viral domains.

• DOI: 10.1371/journal.ppat.1009074
• 发表时间: 2021-01
• 期刊: PLoS pathogens
• 影响因子: 6.7
• 作者: Duddy OP;Bassler BL
• 通讯作者: Bassler BL

An autoinducer-independent RhlR quorum-sensing receptor enables analysis of RhlR regulation

独立于自诱导剂的 RhlR 群体感应受体能够分析 RhlR 调节

• 发表时间: 2019
• 期刊: PLOS pathogens
• 影响因子: 6.7
• 作者: McCready, A. R.;Paczkowski, J. E.;Cong, J-P.;Bassler, B. L.
• 通讯作者: Bassler, B. L.

The intragenus and interspecies quorum-sensing autoinducers exert distinct control over Vibrio cholerae biofilm formation and dispersal

• DOI: 10.1371/journal.pbio.3000429
• 发表时间: 2019-11-01
• 期刊: PLOS BIOLOGY
• 影响因子: 9.8
• 作者: Bridges, Andrew A.;Bassler, Bonnie L.
• 通讯作者: Bassler, Bonnie L.

Mechanical instability and interfacial energy drive biofilm morphogenesis

• DOI: 10.7554/elife.43920
• 发表时间: 2019-03
• 期刊: eLife
• 影响因子: 7.7
• 作者: Jing Yan;Chenyi Fei;Sheng Mao;Alexis Moreau;N. Wingreen;A. Košmrlj;H. Stone;B. Bassler